A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
25 July 2024 9,310 3 View
I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...
24 July 2024 4,720 3 View
I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...
24 July 2024 5,275 0 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
24 July 2024 8,036 1 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
24 July 2024 1,311 5 View
A vegetative state is caused by having massive damage to the mesencephalon, which can include both the pontine nuclei and the thalamus (Plum and Posner 1980). During this state, the metabolic...
24 July 2024 6,127 0 View
I am currently working on a regression model for a project and considering using both Random Forest and Decision Tree algorithms. Given that Random Forest is essentially an ensemble of Decision...
24 July 2024 4,283 3 View
In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.
23 July 2024 3,942 4 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
23 July 2024 136 4 View
Dear All, Can somebody share a diversity and inclusion questionnaire for the business context or military context. Thank you Anuradha
23 July 2024 1,288 4 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
22 July 2024 5,108 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
22 July 2024 8,637 1 View
I want to assign a DOI to works published on Research Gate. I thought Research Gate assigned them, but I don't see how.
21 July 2024 1,547 0 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
21 July 2024 3,368 2 View
Dear Pe
21 July 2024 9,675 6 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
21 July 2024 5,380 2 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
20 July 2024 5,215 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
19 July 2024 5,198 3 View
I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
18 July 2024 8,312 1 View
Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...
18 July 2024 3,440 3 View
I am designing research related to atherosclerosis in vitro, but unfortunately the cell lines currently available in our laboratory are only 3T3-L1 and H9C2, Does anyone can suggest me what...
18 July 2024 6,360 1 View
Hello, how can I do the statistics associated with genotypes and alleles as in the table? thank you very much for your attention.
18 July 2024 7,585 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
17 July 2024 9,028 10 View
Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
17 July 2024 4,680 4 View