A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I am doing in-vitro transcription by T3 and T7 RNA polymerase to make a probe for ISH, but not getting any RNA product on 1.5% TBE gel. I am using a single digested pBluescript vector containing...
27 May 2022 2,165 3 View
Does anyone know how I can perform validation of POLE mutation detection ? Is there any PCR / sequencing assays or positive controls that I can use/purchase ?
26 May 2022 2,991 0 View
During RNA/nuclei isolations we usually have DTT or Beta mercaptoethanol in our buffers which basically reduces the disulfide bonds of the RNases thereby inhibiting them. My question is - since...
25 May 2022 5,063 1 View
I am working to measure the cellulolytic activity of fungi from leaf litter. The clear zone formations form perfectly but the dye surrounding it's circumfernce fades away and I'll say not properly...
25 May 2022 3,521 0 View
The Bioanalyzer basically looks at the quality of rRNAs and generates the RIN value.. Is there a way to access the quality of mRNA or even pre-RNA?
24 May 2022 8,862 2 View
Both Beta-mercaptoethanol and DTT are needed by RNases for stability, thereby inhibiting RNase activity and preserving mRNA. But which one is better and do they both act in the same way?
24 May 2022 5,613 1 View
Dear all, I am planning to do in vitro transcription from a PCR template. However, I need a poly(A) tailed RNA product. If I add 30 nucleotide poly(A) tail before the in vitro transcription,...
23 May 2022 147 3 View
Hello everyone, Can someone help me with this. I have run a complex with DNA, Protein and RNA in NAMD for 50 ns. However, after the end of simulation, the base pairing of DNA and DNA RNA hybrid...
20 May 2022 6,090 1 View
I did the RNA extraction from mice's liver three times over, and the concentration is around 1000ng/ul.(260/280 is 1.9-2.1),then i used 500ng to make cDNA, but when i use taqman probes to do the...
20 May 2022 1,388 3 View
Hi, I want to perform a proteomics study on the organoids which are grown embedded in the Matrigel. I am looking for a defined protocol to isolate proteins from these organoids which helps get...
20 May 2022 5,342 2 View
I wanted to extract total RNA in plant fruit tissues which is abundant of polyphenol and polysaccharide in a high yield (about 100-200 ug). Traditional CTAB and TRIzol method were not suitable...
19 May 2022 6,666 6 View
Can anybody suggest to me any tool or web server, that can predict a probable mutational hot spot?? A big Thank you in advance. :)
19 May 2022 3,110 2 View
Hello everyone, I am trying to conjugate Streptavidin to Silicone which has already been conjugated with the carboxyl group ( COOH), I guess the first question is can I bind Streptavidin to...
19 May 2022 4,575 0 View
I've grown my influenza straind in MDCK cells and have isolated them. Now I have to sequence my virus, my lab uses Eton Biosciences. Do I send in primers for each gene? Do I extract RNA from the...
19 May 2022 2,587 0 View
We are trying to extract RNA from stem cells seeded using 0.5% collagen type 1 into bioactive glass (BGC) scaffolds. We did the extraction twice: 1. First, we added TRIzol to the scaffolds and...
13 May 2022 3,162 8 View
I need to process a large number of 100ml samples through two sets of filters (0.3 and 0.22 micron) and save both filters and the filtrate. While I could do this iteratively with a vacuum or...
13 May 2022 4,628 0 View
Is DNase treamtment is required to the RNA samples isolated from RNeasy mini kit from qiagen before doing one step real time pcr? Thanks
11 May 2022 4,640 1 View
I have a stubborn melting peak in my NTC line when I do probe-base one-step qpcr melting curve analysis for detection of RNA. After I did some control experiments, I found this issue is because...
10 May 2022 8,835 3 View
We recently used QIIME2 to perform bioinformatics analysis on the results of PCR amplification and sequencing of 16S rDNA, and I found Daphnia_magna as one of the species assigned as shown below:...
07 May 2022 4,739 2 View
Hello everyone, I've just started studying about STAR aligner and I came across primary assembly and patch release. I understood so far that patch release is a minor version of a genome which...
05 May 2022 3,412 3 View
We have a hybridization-based, TruSeq-compatible targeted enrichment protocol from Twist Biosciences that we have been using in our lab with great success. We would like to use the Illumina® DNA...
02 May 2022 9,739 1 View
I have LCMV RNA samples of unknown concentration and I want to know how many LCMV RNA copies/mL I have in each sample. To do so, I am going to use the same volume of RNA/sample to perform a One...
02 May 2022 8,950 3 View
Dear all, Two variables: - Expensiveness - Time consuming Using either iTRAQ (protein) or RT-qPCR (RNA) for quantitative (with high sensitivity) expression analysis of a group of 5-15 genes I...
30 April 2022 8,593 2 View
Im using mMESSAGE mMACHINE T7 ULTRA Transcription Kit to transcribe my DNA. It produced 30 ng/ul which is 750 ng although the kit promises to yield atleast 15-20 ug of RNA. Even the control DNA...
29 April 2022 6,878 1 View