Do you know any online database containing pictures (and explanation) about dry bone histology?

I'm interested in both pathological and normal histology.

15 January 2019 3,823 3 View

Why are E. coli strains used for overexpression of recombinant proteins often recA+ as opposed to recA-?

Many molecular biology E.coli strains are recA- to prevent recombination of the plasmid with the host chromosome, or scrambling of the plasmid, and/or mixing of more than one plasmid if...

09 May 2018 7,146 8 View

RNA isolation after TTC staining

How much the staining affects the RNA isolation and proteomic test after using the TTC stained heart tissue?

26 June 2017 9,924 2 View

No title

09 March 2017 3,472 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

I need help converting a PAUP block into a MrBayes block - please help?

18 April 2016 8,561 1 View

Clinical Phase II to Phase III failures in oncology?

I am requesting the help of the RG community to provide me with recent (no older than 5 years) articles (from very high and only from very high ranked journals) clearly 1) indicating how many...

11 February 2016 4,471 18 View

How do you interpret a KEGG/KAAS output?

I've just run a KAAS analysis on my transcriptome. I'm a little unclear as to how to understand the output. For example, I have clicked on "Carbon Metabolism" and this image comes up (see...

21 April 2014 3,775 2 View

Has anyone used breath-easy membranes from Diversified Biotech for growing Chlamydomonas in 24-well plates?

We have tried and our cultures keep dying. Has anyone had this problem?

10 November 2013 4,297 0 View

Increasing/decreasing fluorescence when measuring RNA concentration?

When we measured RNA concentration using fluorescence method with Modulus Turner Biosystems and measured the same sample a few times in a row without opening the lid, sometimes the fluorescence...

05 September 2013 2,591 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View