A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...
26 June 2024 7,512 1 View
Thanks for support
26 June 2024 4,452 0 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
25 June 2024 2,581 3 View
I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.
23 June 2024 7,524 2 View
We are having trouble getting the settings right on a FACS Canto to see a nice population of cells.
21 June 2024 4,111 0 View
I am getting problem to exactly identify the SNP position.
19 June 2024 8,159 2 View
I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...
18 June 2024 8,670 0 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
18 June 2024 6,673 1 View
Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...
18 June 2024 8,533 2 View
Hi all, I mean chromogenic methods.
16 June 2024 4,646 0 View
details of agile diagrams in a flow chart
13 June 2024 4,397 1 View
I don't have 1.5M tris HCl buffer for the resolving gel but I have 1 M tris HCl buffer with pH 8.8 will that work for SDS PAGE? do I need to change the amount added to the mix?
12 June 2024 5,727 3 View
I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...
10 June 2024 9,847 0 View
I want to realize a auto-analyze process for weld fatigue by fe-safe software. However, I found that the current.macro in Tools can not record the commands of the GUI operation about defining the...
05 June 2024 5,067 1 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
02 June 2024 860 1 View
How does biodiversity play a major role in maintaining the ecosystem and role does biodiversity play in the resilience of ecosystems?
01 June 2024 7,148 4 View
Cloud Computing
31 May 2024 2,516 2 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
29 May 2024 3,504 1 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
29 May 2024 6,024 2 View
Hello fellow researchers, Why don't we see two rRNA peaks as is standard? Our sample was a total RNA prep from A549 cell line. We ran our samples on a pico chip for 2100 Agilent bioanalyzer...
29 May 2024 7,643 0 View
Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
27 May 2024 9,284 3 View
In what ways is agriculture biodiversity useful to Indian farmers and why is agricultural diversification essential for a sustainable livelihood?
27 May 2024 9,527 10 View
Hello everyone, I am facing a problem when making a plot in "R". I am generating a ROC curve, but in the graph I have been oberving that my "0.0" scale of of X-axis is far from "0.0" scale of...
26 May 2024 346 1 View
Hi, are there other methods beside Western Blotting to detect a specific protein? I‘ve isolated mitochondria and want to show that hPar17/Pin4 is present in mitochondria. The Western Blots didn’t...
25 May 2024 5,478 3 View