A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Hi all, can anyone help me on how to increase the yield of RNA extracted from FFPE breast tumours tissues. Am using Recover all total nucleic acid kits from Ambion.
02 March 2022 3,530 3 View
Hello everyone, I was running my 10% gel, I ran it on 90V for 30 minutes followed by 120V for around 1:20, then used semi-dry transfer to nitrocellulose membrane. When I developed my blot, I got...
27 February 2022 5,408 0 View
Hi I am interested in IF staining macrophages in frozen colon sections. I chose F4/80 as it is a pan-macrophage marker. However, I have hard time staining macrophages in mouse colons. Typically,...
27 February 2022 5,918 2 View
I want to conduct molecular docking and dynamics of silver nanoparticle (AgNP) with protein. Is it possible to make a silver nanoparticle model using ChemDraw? Do you have any suggestions to do...
24 February 2022 8,376 4 View
My work is with highly plastic eukaryote taxa, with real time visible structural variation on individuals. I suspect they have high epigenetic fluidity while growing, and would like to understand...
22 February 2022 5,574 2 View
Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...
22 February 2022 5,760 1 View
The quality of my gel has been progressively getting worse. I am only attempting to check the quality of the DNA, so even in an example such as this, I can still confirm the topmost band that I...
21 February 2022 9,318 1 View
I'm doing a preliminary experiment to induce CXCL9 via TNFa in Endothelial cells. The plan for the experiment is to bring up some Endothelial cells and expose them to TNFa (as we already have this...
20 February 2022 6,474 2 View
Dear colleagues and fellow researchers, I am planning an experiment and I was wondering if I can use Streptactin (beads) for the pull-down of biotinylated proteins. Obviously, it is cheaper than...
16 February 2022 5,201 4 View
I want to detect Ire1-alpha in fixed, adherent human cells. Could you recommend a reference for which you validated signal specificity with a knock-down or knock-out of Ire1? Many thanks!
15 February 2022 7,824 2 View
I have been trying to isolate total RNA from cattle whole saliva samples for gene expression studies. Saliva samples were collected early morning before feeding to the animal and collected in...
14 February 2022 1,916 5 View
I am doing an experiment where I have to treat the organoids with insulin, harvest RNA and then perform RNA-seq. But I am not sure how long should the insulin treatment be to see an effect on...
14 February 2022 2,483 2 View
So far we have been using SybrSafe for staining agarose gels for dsDNA. Now, we want to produce ssDNA and we are wondering what dye is the best dye for this. On the web we have seen that GelRed,...
11 February 2022 4,551 0 View
To study the milk protein synthesis in the cells I culture primary bovine mammary cells on top of a Matrigel layer in a transwell system, so my cell yield is not very high and also the cells are...
09 February 2022 6,175 0 View
Hi, I am currently dealing with extremely low amounts of environmental microbial RNA (typically
08 February 2022 5,028 9 View
We need to quantify percent parasitemia in a blood sample.
06 February 2022 4,630 11 View
Hi everyone! I am planning to perform a pull-down experiment in order to isolate biotinylated oligonucleotides using srteptavidin-coated magnetic beads. Since I have never performed this kind of...
02 February 2022 7,245 1 View
After one harvests a mouse spleen and isolates the NK cells (through a commercial kit, for example) roughly how many NK cells can one expect to be left with? If anyone has rough numbers they can...
29 January 2022 5,445 2 View
Hi all, I hope this question is not too naive, as i am new to RNA research: I am currently trying to detect putative microRNAs by Northern Blot via radiolabeling (predicted in silico by sRNA...
28 January 2022 8,948 1 View
I do phenol-chloroform RNA extraction. But after running the samples on gel electrophoresis, I see more DNA. What should I do to reduce genomic DNA during RNA extraction?
27 January 2022 6,584 8 View
I have isolated cells from muscle tissue, culture them in adherent flasks and want characterize them. CD56 is a marker for myoblasts, but also for natural killer cells and macrophages. Is it...
26 January 2022 5,324 2 View
Dear all, I am planning to make IVT with a plasmid I already have. The plasmid is composed by: ..........T7 promoter - cDNA of my gene - P2A - eGFP - bGHPolyA ............ Looking in the datasheed...
25 January 2022 1,285 7 View
I am trying to purify a single-pass transmembrane protein from 293F cells. I have just started working on membrane proteins, so I have limited experience. Could anyone share a detailed protocol...
25 January 2022 1,533 6 View
Theoretically, Stage III includes IIIA, IIIB, IIIC. So, I wonder why is there a fourth group (Stage III) in the Subtype profile workflow at Gent2 database. My other question does anyone know a...
24 January 2022 5,113 2 View