Is running several one-way ANOVAs justifiable when an (appropriate) two-ways ANOVA does not reach statistical significance?

Hi, We have been discussing this topic on my lab for a long time. Is there any justification for reporting statistically significant results of several one-way ANOVAs when a previous and more...

29 January 2020 8,458 2 View

Choosing compound' doses for rats when only mice-related bibliography is available?

Hi all, We are planning to systemically deliver a synthetic pharmacological compound in rats. When looking for specific dosage and administration protocols, we only found studies with mice....

05 May 2019 3,644 5 View

TCID50 for Non-forming and Non-CPE virus?

Is there a possibility to calculate TCID50 for non-plaque forming virus, the virus I'm trying to titer is also known to be non-cytopathic, so my questions is: Is there a method of titers this sort...

15 April 2019 8,266 2 View

No title

10 March 2019 7,613 4 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

Can someone help me with Immunofluorescence Assay for CSFV quantification?

I'm testing a protocol for viral quantification by IF; it is being standarizing from the fixation step, as a fixative choosen are Methanol (10mins) and 4% Formaldehyde-10%Methanol (15mins) (Is...

26 February 2019 3,020 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

I need to extract dna from environmental biofilms from contaminated sites that solution recommend me to clean my samples?

biology

03 April 2017 9,126 0 View

Somebody can recommend me some Books?

I'll become a student (MSc by research in organic chemistry). I would like to know some useful books expecially for the "work in the laboratory" like spectroscopy, how to purify some reagents...

02 March 2017 2,429 0 View

What is the best way to recover low amounts of DNA from an agarose gel band?

10 November 2015 9,428 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View