A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Referee rewiever
01 May 2024 3,852 7 View
From clinical sample we are trying to identify Staphylococcus aureus by using nutrient agar. Based on the appearance of colonies on the nutrient agar plate please describe the characteristics that...
30 April 2024 9,928 5 View
The truth is that I have had many, many problems to be able to see a specific band of this protein. My problems have ranged from bad transfers, to unknowns that I have not been able to answer. The...
27 April 2024 8,524 4 View
Dear Community, I recently performed a Western Blot to test whether the mitochondria marker ATP5A1 is in the experimental cells or not. However, the results did not show up at the expected MW . I...
25 April 2024 2,040 1 View
Hi! I´m currently trying to isolate Mitochondria from HCM cells. For my western blots I need an additional antibody against a mitochondrial protein which is bigger or smaller than 14/17 kDa. I was...
25 April 2024 8,785 2 View
After treating my membrane with the primary antibody, the destaining buffer was accidentally used instead of tbst buffer. Immediately after use (about 3 seconds later) it was replaced with tbst...
25 April 2024 2,595 1 View
i am refolding protein by gradient dilaysis in which i gradually decrease concentration of urea by gradient dialysis. however im not getting surety wheather denaturant has been completely removed...
25 April 2024 5,379 1 View
I have no idea why my orthotopic mouse models have failed. I have used gastric cancer cell lines, and I injected the cells into a mouse stomach. Here is my procedure and a result photo as a failed...
24 April 2024 1,885 9 View
Hi, if someone has data that are scored: 1 for poor, 2 for adequate and 3 for good. Can you take a mean average if a score of less than 1 is not possible? I'm thinking if a mean score of...
24 April 2024 6,337 1 View
Do these processes involve a complex interplay between molecular signaling pathways, regulation of gene expression, protein modifications, and cellular interactions? Could dysregulation of any of...
23 April 2024 9,247 3 View
Dear all, I am looking for a protocol for neuraminidase (NA) treatment of adherent A549 cells (as a control for SNA and MAL-II staining). I am particularly interested in: - Did you fix the...
23 April 2024 9,904 1 View
Looking for most cost-effective manner to outsource my samples (silver stained gels) for proteomics analysis using LC-MS/MS. If anyone has outsourced samples outside or in INDIA for LC-MS/MS and...
22 April 2024 288 2 View
We have assembled a chloroplast genome of angiosperm and submitted the annotated assembly to NCBI GenBank. Within two-working days we got accession number for that submission. GenBank in their...
22 April 2024 9,949 0 View
I am using sybr green mastermix and my cDNA quantity is okay. Still not getting amplification curve both for expected gene and internal control (GAPDH)
21 April 2024 9,863 5 View
Hi everyone, I'm working on a project where I need to compare the similarity between line curves on two separate charts, and I could use some guidance. Here’s the situation: First Chart Details:...
20 April 2024 4,332 9 View
I would like to do genome assembly for the bacteria isolate from the environment. Can any provide me the information or any tutorial? It would be helpful! thanks!
19 April 2024 1,863 12 View
I'm docking an enzyme by using maestro, there is an metal locate at the docking active site, I have already tried a few methods but there are still show the metal is interact with ligands in...
19 April 2024 3,978 4 View
Why is energy not cycled through an ecosystem and one way flow of energy and the cycling of materials important in the ecosystem?
18 April 2024 708 3 View
I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...
17 April 2024 3,512 2 View
For validation by qPCR of several microRNAs, is it necessary to measure normalization miRNAs in each plate in addition to the miRNAs under study? Or can you make plates of microRNAs and then...
16 April 2024 6,361 1 View
Hi all, I'm trying to isolate zebrafish embryonic stem cells after 3 hpf of zebrafish embryo's. I have a couple of questions. - I already tried Pronase to dechorionate, but it is very difficult....
16 April 2024 9,026 0 View
Hi, I have used masson's trichrome staining and trying to analyze collagen staining.. How can we analyze it using Image J. Anyone have the macros available? Thanks
15 April 2024 5,572 2 View
We have developed an orally delivered compound that elevates serum nitric oxide where the NO has a circulating half-life of ~5 hours.
15 April 2024 6,523 2 View
The lanes contain the same sample and concentration (15ug). The image contains GluN2A, PSD95, and Beta Actin (Top, middle, bottom respectively). Due to this being the same sample every lane should...
12 April 2024 4,014 2 View