A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
i have do the EMSA assay, and can see weak protein-RNA complex band, the band is so weak that we can't use the sample by mixing the protein and RNA directly for Cryo-EM study and determine the...
22 November 2021 6,415 2 View
What will be the ideal statistical test to compare fasting and random lipid levels in a nationwide cross-sectional study?
21 November 2021 9,458 7 View
Hi, everyone! There is a problem about CuAAC efficiency that troubles me a lot. I modified a water soluble comb-like copolymer polyallylamine-graft-PEG (PAA-g-PEG) with azidoacetic acid (about 15...
21 November 2021 8,994 6 View
I follow the below protocol to make 4OH tamoxifen in corn oil. 1) 50 mg tamoxifen + 2.5 ml of 100% ethanol (add in the same amber bottle in which Tamoxifen comes) 2) Keep the amber bottle at 37C...
19 November 2021 8,710 1 View
Hi, I have been developing a BONCAT protocol for use on environmental marine bacterial samples using L-azidohomoalanine (AHA). From my testing and literature research, I found that incubating...
15 November 2021 7,037 0 View
I want an suitable alternative
15 November 2021 1,983 1 View
I am interested in extracting DNA/RNA/Protein at a high-purified level from cardiovascular and skeletal muscle tissue. RNA samples will be used for performing gene expression using RT-PCR,...
12 November 2021 2,051 1 View
Hi. We are planning to do a single-cell RNA seq in combination with an ONT system for sequencing instead of Illumina. So is there anyone who has done it before? There are a few steps in the...
10 November 2021 5,284 4 View
I am starting to investigate cell migration (A549 cell line; wound healing assay) for longer than 24 hrs. I want to make sure I am looking only on migrating but not proliferating cells. Are there...
09 November 2021 1,176 2 View
I am new in the field of plant nutrition and I am searching for the most relevant markers to use to follow N and P starvation in Arabidopsis (both in shoots and roots). What are the most sensitive...
09 November 2021 8,109 3 View
I have been extracting RNA via Qiagen minikit RNA extraction kit, and the conc are looking good on nanodrop (colibri) but the A260/A280 ratios are consistently shown high, in ranges of 3.6 or...
09 November 2021 5,005 12 View
Hello, I am going to to do deletion in 286bp in the promoter region using CRISPR Cas9, How many guides in the construct will give the ideal result?
08 November 2021 7,238 6 View
I am performing a cloning experiment of a plasmodium gene with pUC-18 vector in E.coli (strain DH5alpha). After ligation when I am trying to analyze my vector+insert plate (X-gal+IPTG+LB-Amp), I...
30 October 2021 6,998 0 View
Hi everybody! I'm a student looking fore some suggestions on how to stimulate in a TCR-Dependent manner my cells to assess early phosphorylations (SLP-76, LAT and SHP) in TCR signalling cascade...
27 October 2021 1,613 0 View
Currently I am plating human monocytes at a concentration of 1million/mL in a 48 well-plate where I add 400,000 per well. I differentiate them by adding MCSF (100ng/mL) for 6 days (change media on...
26 October 2021 7,860 5 View
Hello everyone, I'm currently investigating the model diversity of transmission electron microscopes used in biological research. Trying to find out which producer/model would be the best solution...
25 October 2021 9,590 3 View
Hello all, For a brief period (7-10 days) someone had accidentally stored our supply of sybr safe ( invitrogen: S33102) into our -20 freezer compared to keeping it at room temp/ in a fridge. Has...
25 October 2021 1,079 0 View
Hi, I have been trying to record Parvalbumin interneurons in mouse brain slices. I tag the PV cells with AAV. However, most labeled PV cells are not on the surface of the slices. I'm having...
25 October 2021 5,342 1 View
I am new to Desmond simulations and I want to know how can I find the estimated time left for a simulation to be completed? my 2nd query is how to perform B-Factor analysis after performing...
20 October 2021 1,612 0 View
Hello all, I was wondering if there is a negative effect of RNase inhibitors (like RNase Out, RNasin, etc) on low input RNA samples at higher concentrations? I find that many single-cell RNAseq...
20 October 2021 5,971 2 View
I am new to this field. I am interested in learning the best practices to study a particular gene's mRNA isoforms present in cultured mammalian cells. I want to detect mRNA transcripts containing...
19 October 2021 1,142 0 View
We wish to buy a multifunction microplate reader (Absorbance, fluorescence, luminescence))))) and we have contacted TECAN, Perkin Elmer and Molecular devices. I would like to know if someone had...
15 October 2021 4,279 0 View
I want to make 4-mer, 6-mer or 10 mer of my recombinant protein. I may like to go even higher multimers as long as it is controlled and defined. Is there a way to go about it apart from the...
14 October 2021 7,550 3 View
Hello, I would like to know how to induce in vitro apoptosis on neonatal cardiomyocytes ? Except putting the cells in hypoxia. Thank you in advance for your help.
13 October 2021 2,498 4 View