A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
29 July 2024 3,910 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
29 July 2024 8,346 2 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
28 July 2024 8,902 0 View
In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.
28 July 2024 2,637 2 View
I would really appreciate it if anybody could help me with my problem. I’ve been working on the macrophage Raw 264.7 cell line for 5 months. Despite following all the advice from ATCC and other...
28 July 2024 9,211 7 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
26 July 2024 6,725 3 View
What roles do microbial communities play in the environment and roles played by microorganisms in our environment?
26 July 2024 2,153 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions？
26 July 2024 5,398 2 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
26 July 2024 7,833 0 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
25 July 2024 8,652 2 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
25 July 2024 2,579 2 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
25 July 2024 10,040 3 View
How far is the distance with question marks on it? There is a river, where you measure at the point out to the right and there is a low screen. The river is green, what is visible is within the...
25 July 2024 4,600 1 View
Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...
25 July 2024 8,573 5 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
25 July 2024 9,531 3 View
I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...
24 July 2024 4,839 3 View
I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...
24 July 2024 5,413 0 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
24 July 2024 8,283 1 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
24 July 2024 1,710 5 View
A vegetative state is caused by having massive damage to the mesencephalon, which can include both the pontine nuclei and the thalamus (Plum and Posner 1980). During this state, the metabolic...
24 July 2024 6,354 0 View
I am currently working on a regression model for a project and considering using both Random Forest and Decision Tree algorithms. Given that Random Forest is essentially an ensemble of Decision...
24 July 2024 4,477 3 View
In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.
23 July 2024 4,333 4 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
23 July 2024 408 4 View
Dear All, Can somebody share a diversity and inclusion questionnaire for the business context or military context. Thank you Anuradha
23 July 2024 1,496 4 View