A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
4,383 views 5,329 posts
Questions related to RNA
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
15 July 2024 355 4 View
I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...
14 July 2024 7,966 2 View
I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...
14 July 2024 725 1 View
When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...
14 July 2024 7,251 0 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
14 July 2024 2,588 3 View
What ethical dilemma is in predatory journals and difference between open source and closed source?
13 July 2024 7,600 2 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
12 July 2024 7,996 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
12 July 2024 3,611 3 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
12 July 2024 8,018 3 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
12 July 2024 1,860 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
12 July 2024 4,963 0 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
12 July 2024 4,489 0 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
12 July 2024 5,119 5 View
I would like to know how to check if a given research work is human made or AI generated?
12 July 2024 8,696 5 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
12 July 2024 2,306 1 View
I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?
11 July 2024 2,647 1 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
10 July 2024 4,343 1 View
Can
09 July 2024 6,791 4 View
I am a beginner in this field. While I am reading papers and the understanding is getting better. However can someone explain in easy and simple words. Really thankful !
09 July 2024 8,447 5 View
I have hemostatic nanofibers of hydrophilic nature. While fibrin formation from fibrinogen can be qualitatively visualized using SEM. What assays can be performed on nanofibers to quantify the...
09 July 2024 3,761 1 View
Data Acquisition detected more than 65000 metabolites with area maximum, RT-value and peak-rating. What is the significance of Peak Rating?
08 July 2024 6,753 2 View
Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...
08 July 2024 9,685 1 View
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
08 July 2024 3,026 1 View
What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...
07 July 2024 3,013 0 View