A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
What are the different types of crossover operators in GP?
30 March 2024 7,814 2 View
I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...
30 March 2024 5,946 1 View
How to apply blinder Oaxaca decomposition methods to decompose the impact of two or more factors on a dependent variable on different Social groups?
29 March 2024 7,255 1 View
I have a question for the professionals. The essence of the problem: I fix the oligos on a plastic substrate, they play role of primers in solid-phase PCR. I carry out a one-step PCR with...
29 March 2024 9,092 2 View
While observing the protein expression of cells with a confocal microscope, I accidentally found a microstructure that I had no clue about. The colors in the picture are cyan for the nucleus, blue...
28 March 2024 7,188 2 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
28 March 2024 8,249 0 View
Why is CS655 soil moisture sensor reading Soil water content (SWC) as 6999 or -6999
27 March 2024 1,451 0 View
I am currently trying to do chromosome counts on algal cells. However, I don't have the reagents necessary to extract full protoplasts. But I can make small holes which can allow the DAPI stain to...
26 March 2024 4,782 2 View
I would like to expand memory B cells from a single memory B cell, and we are working on establishing hCD40L expressing -3T3 or -293T cell lines. Before we get the hCD40L-cell lines, we have tried...
26 March 2024 5,056 0 View
Are all microalgae capable of producing biofuels, and how do they do it and best microalgae strain for biodiesel production?
26 March 2024 962 5 View
How microalgae clean wastewater and microalgae do based technologies for circular wastewater treatment?
26 March 2024 9,890 4 View
Hi all, Kindly I have questions about peptide design. I'm now in the process of designing a peptide based on protein structure and the length of peptide is 45 a.a . I have predicted the structure...
24 March 2024 5,210 1 View
What is the maximum yield of colanic acid has been produced? and What is the best method of producing Colanic acid? Tell me the process, production, purification, characterization, and removal...
24 March 2024 9,013 0 View
In some gene editing studies using mice, the delivered plasmid carrying the editing tool (e.g. TALEN) also carries both an HA-tag and GFP. The GFP is separated from the editing tool with a T2A...
23 March 2024 1,456 3 View
How does elevated carbon dioxide affect the ecosystem and an increase in the microorganisms in the soil affect the soil quality?
23 March 2024 9,421 2 View
Any information about this?
21 March 2024 1,450 0 View
I am working with suspension cells, JY cells in particular. I have been using hypotonic lysis (buffer components: 10 mM Tris-HCl pH 7.4) to attempt to obtain cytosolic and membrane protein...
19 March 2024 6,518 4 View
Months ago i was able to induce NETosis in Hl60 differentiated cells using PMA while now with a different PMA solution I can't anymore. I followed the condition reported in literature of 100nM PMA...
19 March 2024 2,899 0 View
It is seen that a nation is born with a religious essence, but some nations do get birth depending on the strength of cultural aspects. But religion influences the presence of cultural identity a lot.
19 March 2024 8,334 4 View
I tried to isolate pr-FMN from UbiD like enzyme and verify it via UPLC and Mass spectrometry. The results obtained from MS shows detection of right mass but UPLC spectrum tell another story. How...
18 March 2024 4,628 3 View
I'm using Jurkat E6.1 cell line, kindly recommend what media should I use; at present I have RPMI-1640 without sod pyruvate & 2g/L glucose, is it ok?
18 March 2024 4,715 3 View
I recently performed an RNA FISH experiment with five labels: Hoechst, GFP-tagged protein, Quasar570 RNA probe, mCherry reporter, and Quasar670 RNA probe. All probes are essential for the...
17 March 2024 9,761 0 View
I have several scRNA-seq datasets of tissue immune cells obtained from FACS sorting. When analyzing the data, I find when a dataset is rich in myeloid cells (e.g. macrophage), there tends to be a...
14 March 2024 9,444 0 View
How does genetic diversity promote survival of the species in the ecosystem and ecological importance of biological diversity in climate resilient agriculture?
12 March 2024 4,446 2 View