Hello! I posted a similar question a few days ago, but I accidentally typed in the wrong concentration & I am unable to delete my old question. I sincerely apologize for the repetition.
I am having a lot of trouble trying to calculate the concentration needed to make a 50 mL DMEM media + 2.5mM 2-Deoxy-D-glucose (2-DG) solution for my cell culturing experiment.
For reference, the media I am using is a DMEM 4.5 g/L glucose - https://ecatalog.corning.com/life-sciences/b2c/US/en/Media%2C-Sera%2C-and-Reagents/Classical-Media/Dulbecco%27s-Modification-of-Eagle%27s-Medium-%28DMEM%29/Corning%C2%AE-DMEM-%28Dulbecco%E2%80%99s-Modified-Eagle%E2%80%99s-Medium%29/p/10-013-CV
The molecular weight of 2-DG is 164.16 g/mol. The 2-DG that we have in the lab is a crystalline powder. I know that dissolving 1 g of 2-DG in 1 L of media makes a 1M solution, but I need assistance with the next calculation steps.
Hello Kirsten Navarro,
The calculation for 2.5mM instead of 50mM is as follows
For 50mM 2-DG, I had mentioned that you will have to weigh 410mg of 2-DG powder and dissolve in 50ml DMEM.
So, for 2.5mM 2-DG, which is 20 times of a lesser concentration than 50mM, you will have to weigh 20.5mg of 2-DG powder and dissolve in 50ml DMEM to make a 50 mL DMEM media + 2.5mM 2-Deoxy-D-glucose (2-DG) solution.
Best,
Hi Kristen, to prepare 50 mL of a 2.5 mM 2-DG solution, given that the molecular weight of 2-DG is 164.16, the required amount of 2-DG (in mg) is calculated by multiplying the concentration (mM), volume (mL), and molecular weight, which equals 20.52 mg. It is highly recommended to use useful online tools, such as the molar calculator on the MCE official website. You can directly input the desired concentration and volume into the calculator, and it will calculate the required mass of 2-DG powder. For example, dissolving 1 g of 2-DG in 1 L of culture medium will result in a 6.09 mM solution.
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