Flash freezing in liquid nitrogen and storage at -80C should work.
Thanks a lot for your answer!! I don't have at this moment liquid nitrogen. I was wondering if it could be work using trizol for preservation? I have also read RNAlater solution, but I'm kind of hesitant about this last one.
Hello Gabriela J Perez
The method stated by Melissa Chernick is the best. But since you don't have liquid nitrogen, I would like to suggest an alternative.
RNA later is a preservative which stabilizes cellular RNA without the need to freeze the samples. This solution preserves intact RNA by precipitating out RNases into an aqueous sulfate salt solution.
On the other hand, TRIzol can only inactivate RNases with which it is in direct contact. Therefore, the organs are not safe from RNA degradation until it is completely homogenized.
So, I would suggest you use RNAlater for the storage of zebrafish organs for future RNA extraction.
Completely submerge the organ in the collection vessel containing RNAlater. With RNAlater, organs can be stored at room temperature for up to 1 week, at 4 deg C for up to 1 month and at -80 deg C for a long time. RNA extraction may be done later using TRIzol.
Organs that have been stored in RNAlater should be removed from the storage solution with sterile forceps, and submerged in TRIzol for RNA extraction.
Best,
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