I know there are waterdock and dowser programs, however those seems to be not supported verywell. Thanks in advance.
I suggest you to use gromacs.
for sung that install gromacs and run the following commands to get the structure with water:
gmx pdb2gmx -f protein.pdb -o processed.gro -water tip3p
after that enter this :
gmx editconf -f processed.gro -o boxed.gro -c -d 1.0 -bt triclinic
gmx solvate -cp boxed.gro -cs spc216.gro -o solvated.gro -p topol.top
the "solvated.gro" is your file with water in a box 1.0 angestrom distance of each side and solved with water. open this file in pymol or chimera and save in pdb format or each format you want.
The expiration date of my PEI transfection reagent is out and I do not know how to check it. I only use siRNA in my work and I can't figure out what I'm doing wrong or what's wrong with PEi. Can I...
04 December 2023 4,783 2 View
I am performing siRNA transfection to inhibit a certain matrix RNA. Does it make sense in this case to determine the reduction of this RNA by reverse transcription on PCR?
30 October 2023 1,494 4 View
I have a certain misunderstanding with this term, for a literal translation into Ukrainian (English speakers, please, because I don't really understand the context). I look at the photo and the...
05 July 2023 5,056 0 View
Is there an optimal particle size that is suitable for the extraction of metals (iron, titanium, aluminum, REE) from soil? If the particle size is small (
26 June 2023 8,296 0 View
Dear colleagues, Whats your opinion on the top-10 most popular/basic theories in the field of information (technology) and economics? That answer the question "how information collection,...
22 April 2023 8,538 6 View
If it does, how does the situation look for crystalline and amorphous materials? Suggestions of papers/books to educate me on this topic are appreciated.
08 February 2023 2,822 1 View
I need to make low cost reflectance calibration samples. I plan to apply special paints on a plywood board. For high reflectance, BaSO4 paint works well, but I can't find low (10%) reflectance...
07 November 2022 5,059 3 View
Are there indexes for calculating the biological weathering? Or do we have other ways of determining the intensity of biological weathering? It would be appropriate if they were based on the...
26 July 2022 2,775 0 View
A scheme that includes different elements of QKD and their interrelations, like servers, random number generator, photon counters, etc.
14 October 2021 1,533 2 View
In textbooks on astronomy, we can find information that the radius of the habitable zone is influenced by the luminosity of the star, the sperctral class, and metallicity. But are there any...
17 June 2021 7,279 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View