09 December 2016 5 5K Report

Hi all,

I am trying to amplify naked mole rat CD44 from CDNA. Sequence of CD44 is taken from http://www.naked-mole-rat.org/annotations/details/XM_004852041.1/. 

I want to amplify CD44 cDNA. The primers i generated are as follows;

NMR_CD44_EcoRI_F 5` TAAGCAGAATTCATGGACAAGTTCTGGTGGCG 3`

NMR_CD44_EcoRV_R 5` TGCTTAGCGATATCTTACACCCCAATCTTCATGTC

Basically, I want to add EcoRI at 5 ` and EcoRV at 3`.

When I do the PCR with these primers using CDNA, I do not see even a smear, let alone a amplified band. I only see very bright band of primers down of the gel.

I hope the primers are fine.

Any suggestion would be appreciate.

Many thanks

Similar questions and discussions