Hi all,
I am trying to amplify naked mole rat CD44 from CDNA. Sequence of CD44 is taken from http://www.naked-mole-rat.org/annotations/details/XM_004852041.1/.
I want to amplify CD44 cDNA. The primers i generated are as follows;
NMR_CD44_EcoRI_F 5` TAAGCAGAATTCATGGACAAGTTCTGGTGGCG 3`
NMR_CD44_EcoRV_R 5` TGCTTAGCGATATCTTACACCCCAATCTTCATGTC
Basically, I want to add EcoRI at 5 ` and EcoRV at 3`.
When I do the PCR with these primers using CDNA, I do not see even a smear, let alone a amplified band. I only see very bright band of primers down of the gel.
I hope the primers are fine.
Any suggestion would be appreciate.
Many thanks