Is it possible to proceed to rt pcr if i got low 260/280 which is around 1.2-1.4?

More Nabilah Mohamad's questions See All

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

Which statistical analysis do I use for data analysis of a questionnaire?

I am writing a research proposal and I have to analyze my data. My research is about student's time management and its relation to academic performance. I am using Britton and Tesser, Time...

26 December 2020 6,883 3 View

How to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS?

Dear researchers, how to model linear-elastic large deformation of 1D Uniaxial stress in ABAQUS? How does the abaqus distinguish between the 1d uniaxial stress and strain analysis? Thanks in advance.

20 December 2020 370 2 View

Is reconstitution solvents (etc: acetone, methanol) in the last step of extraction important in securing the compounds peak in the HPLC or LC-MS/MS?

I have used different reconstitution solvents in my sample extractions for an example, I used methanol as my reconstitution solvents in the water and sediment extraction sample whereas, I used...

25 November 2020 6,842 7 View

How to formulate or simulate FSI and 6DOF together?

hi every one I want to know, is there any way to formulate FSI and 6 DOF or simulate them together in CFD Softwares? for example FSI of a ship with 6 DOF in regular waves or irregular waves? thanks

01 November 2020 6,197 3 View

No title

Hello there I'm a Ph.D. student in clinical psychology and need to learn R software. I have enough knowledge of SPSS and AMOS. Are these skills enough for starting R software learning for...

23 October 2020 8,220 5 View

How is solubility of CO2 or air in organic solvents such as xylene or cyclohexane?

For some reason, I need to inject a dispersion of an organic solvent and organoclay into a block of soil sample. the organic solvent probably is xylene.

29 September 2020 6,348 4 View

How to decide sample size for tourists based on available tourists arrival data in my case study in Gunung Mulu National Park?

My study will be conducted in Gunung Mulu National Park, Sarawak, Malaysia. The target respondents are local community and tourists. For the local community, there is no problem. But, I am still...

23 September 2020 1,421 1 View

Are there any experimental data on the graphitization effect of neutronics in HTGR?

I'm doing a neutronic simulation of a high-burnup, high-temperature graphite-moderated reactor. Is neutronics significantly affected if graphitization is neglected/considered? Note that I have...

22 September 2020 7,780 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Agustina Lino

the ratio should be >1,8. But you could try and do the Reverse transcription and the PCR for a housekeeping gene (b-actin, GAPDH).

Thilini Keerthirathne

Working with low-quality RNA can alter the experimental results and it is better to use high-quality intact RNA in molecular biological diagnostic applications for better, accurate results.

Emad AL-RADHI

I have same problem I think in purity of RNA