Usually we do it with 6 embryos. Now for a lot of reasons we need to do RNA isolation and even Western blot with only a single embryo. Please kindly guide me with your notes. Thanks
Of course, you can. Do the same procedure as for 6 embryos - only reduce the amount of buffer and solutions you need. (Remember you can isolate DNA from a single cell)
Dear Saeid
Hello,
to perform single embryo transcriptome analyses using zebrafish as a model organism, please go through the protocol introduced by Jong et al. (2010) RNA isolation method for single embryo transcriptome analysis in zebrafish. So you will NOT be confounded by average mRNA level of pool embryos.
Best,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845602/
Thank you Vera and Hadi for your comments. Your answers were really helpful.
Dear Saeid,
I successfully use the protocol of Jong et al. with some adaptations.
Good Luck!
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