How I can do RNA isolation from a single embryo of Zebrafish at day 1 and 2 post fertilization?

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Vera Hemleben

Of course, you can. Do the same procedure as for 6 embryos - only reduce the amount of buffer and solutions you need. (Remember you can isolate DNA from a single cell)

Hadi Bayat

Dear Saeid

Hello,

to perform single embryo transcriptome analyses using zebrafish as a model organism, please go through the protocol introduced by Jong et al. (2010) RNA isolation method for single embryo transcriptome analysis in zebrafish. So you will NOT be confounded by average mRNA level of pool embryos.

Best,

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845602/