Hello,
I'm planning to extract RNA from a sample containing infectious bronchitis virus (gammacoronavirus) purified using a continuous sucrose gradient. Which one of the RNeasy extraction protocols gives better results? Animal cells, animal tissue, yeast or plants and fungi? Or does QIAamp viral RNA extraction kit works better? Any suggestions are more than welcome. The purpose of the extraction is to send it for deep sequencing using Illumina MiSeq. Thanks a lot in advance.
Sibnarayan Datta
Dear Jose,
some years back, I worked with HCV purified by sucrose gradients. I extracted RNA using the TRIZol method and had good quality RNAs for Northern Blots and RPA assays.
Best wishes
SD
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