Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

How to understand the chemo-mechanical coupling?

Recently, I came into contact with a new discipline named chemo-mechanical coupling. I found that chemo-mechanical coupling phenomena exist in many research areas, ranging from development of...

17 December 2020 1,065 3 View

I am working on membrane distillation model in COMSOL and I have some problems coming in it?

I need to add a latent heat flux of evaporation to the hot side boundary, which is a function of the pressure difference between the cold side and the hot side boundary.However, the pressure at...

16 December 2020 9,890 1 View

How to spin coat thin film for PEC devices (improve viscosity)?

I am trying to repeat the fabrication of BiVO4 (BVO) using the metal-organic deposition method. This is a well-reported way to make BVO thin film on FTO. So basically, I just dissolve Bi(NO3)3...

18 March 2020 8,405 7 View

Does anyone know the amino acid sequence of rat NRF-2?

Hi, I'm trying to find a conjugated anti-rat Nrf2 monoclonal antibody for flow cytometry analysis in rat models. Unfortunately, i'm yet to find a conjugate that has been tested in rat samples. The...

03 March 2020 5,541 6 View

Why ecology system allows virus exsit?

27 February 2020 5,293 3 View

Reliable PGP9.5 antibody to visualize intraepidermal nerve fibres in rat paw skin?

Hi, I'm looking for a reliable PGP9.5 antibody that can visualize INEF in rat paw skin. Most publications with good image on rat paw uses Ultraclone or AbD Serotec's rabbit anti-human antibody...

19 February 2020 979 2 View

Differential Scanning Calorimetry (DSC) protein aggregation?

I am working on Differential Scanning Calorimetry (DSC) for my protein. Mainly I want to check its melting temperature and enthalpy changes. I first tried two standard proteins ( BSA and...

17 February 2020 1,733 3 View

Why does DAB staining not work, given that TSA on the same tissue works?

I am staining for c-fos in ferret brain sections using this antibody (https://www.cellsignal.com/products/primary-antibodies/c-fos-9f6-rabbit-mab/2250). The sequence of ferret c-fos is highly...

09 January 2020 4,671 1 View

What gas flow rate should I choose for calcinating CaCO3 in the air ?

A lot of papers talk about calcination temperature, but little of them mention the gas flow rate. I find gas flow has a strong influence of my catalyst... I am preparing an organic Calcium...

18 November 2019 2,879 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View