Rna extraction from breast cancer explants?

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What are the governing equations for "Impact Classifier Mill" in Powder Coating Industry?

In Powder Coating Industry, Is there any equation that relates physical properties of chips (granules of thermosetting polymer, i.e, extrudate that has been cooled and crushed) such as dimensions...

26 January 2021 1,077 3 View

Why should we use Sustainable climate design?

Sustainable design is a set of cognitive and analytical abilities, knowledge of the environment and ample practical to incorporate things into the world of microbes, plants and animals, and the...

25 January 2021 7,816 8 View

Is it possible to adapt all new and old buildings to be environmentally friendly and how can we do that?

An Architecture and Environmental Design degree studies the basic principles behind environmental issues that need tackling through architecture. Sustainable design is the field of creating...

25 July 2020 1,328 17 View

Free Water Hammer Transient Hydraulic applications?

I have found x2 application that fits the above category: 1] Very old 16-bit application but you can run it using opensource emulator otvdm (on github) https://imgur.com/a/aO7ZgXR 2] A modern...

12 April 2020 2,568 2 View

Best Reinforced Concrete Finite Element Analysis Software Package?

Respected colleagues, What is the best finite element modeling software packages for reinforced concrete elements, such as beams, slabs, columns, etc subjected to static monotonic loading? I've...

26 November 2019 2,704 8 View

How to find the PI gain for electrical parameter estimation in sensorless control of PMSM?

I want to know how to tune the PI controller in case of parameter estimation, let say i have equation Ls=[Kp+Ki/2]*[VdEd+VqEq] so how to estimate Kp and Ki value for better performance of the...

31 October 2019 3,691 4 View

What are the proper Boundary conditions for electrolyte and electrode potentials in an electrochemical cell?

Introducing the proper Boundary conditions greatly affects any cfd results. Could any one discuss with me the boundary conditions to be introduced in an electrochemical cell? especially those for...

30 August 2019 8,173 2 View

Final year project on enery crisis?

i am doing electrical engineering.now i am in final year....i need help regarding final year project.....which power project shoud i work on?.....plz suggest me some topics....complete...

26 August 2019 4,159 3 View

What is the relative standard curve method?

The relative standard curve method in qPCR is a standard curve-based method for relative real time PCR data processing. Can anyone explain the calculations of this method to me? I read something...

20 July 2019 5,799 3 View

Simulating the effect of adding an object inside Room Impulse Response generator or MCRoomSim?

Hi All, I am currently working with Room Impulse Response (RIR) Generator and MCRoomSim in Matlab for simulating room impulse response using different signals but is there a way to add objects...

21 March 2019 3,777 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Paola Di Bonito

How many ul did you get? May be it is not a low yield

Omnia Usama

30-20 uL

Eliot Barson

Try freezing thawing at -80oC a few times after the addition of the TRI reagent to stop the degradation of RNA. Trizol has RNAse inhibitors.

You can be greedy whilst taking the initial aqueous phase if you repeat the chloroform step.

This way you can attain all the RNA and reduce the amount of impurities you take forward.