Rna extraction from breast cancer explants?

More Omnia Usama's questions See All

Are there environmental clues from sediment texture?

05 June 2024 2,855 1 View

Why do we predominantly utilize the Caputo sense of AB derivative over the Riemann sense in modeling real-life problems?

1. Additionally, what do we mean by the terms "memory preserving" and "heredity property" in this context? 2. How can we determine the appropriate fractional derivative, or kernel, for modeling...

04 March 2024 4,843 0 View

What is Hypertension Associated with obesity?

Hypertension

02 January 2024 7,671 3 View

Exploring the "I": A Psychoanalytic Analysis of Selfhood and Identity in the Poetry of Sylvia Plath and Anne Sexton?

Please any relevant literature review or helpful materials on the above poets and their poetry. I am using these poems; for Sylvia Plath: - Lady Lazarus - Daddy - Mirror - Ariel - Tulips - The...

01 December 2023 10,004 2 View

Which software is better to study about thermodynamic cycles in your opinion?

I would like to obtain a design program, a free version, available to engineering students for a graduation project. The graduation project is a simple gas cycle and exhaust from it enter to HRSG...

02 November 2023 1,754 3 View

Why is it necessary to provide housing quality indicators?

Housing quality is measured using objective and subjective indicators, as well as quality of life. On the one hand, subjective indicators investigate housing quality at the individual level; it is...

02 October 2023 797 4 View

How can i find the most recent statistics on the infectious diseases, including total number of persons affected and the areas where it is spreading?

How can I find the specific geographic regions in Pakistan where the particular epidemic disease is more pronounced due to the environmental factors?

27 September 2023 6,357 4 View

Is there a relationship between happiness and well-designed projects?

There is no question that an unpleasant working environment has an impact on both our physical and emotional health. Is it possible for good design to make us happy? Given that we spend almost...

18 September 2023 1,931 2 View

Kindly, help me by sharing matlab or mathematica code of fuzzy-valued function. I shall be very thhankful to you?

24 August 2023 8,403 1 View

Please share the MATLAB or Mathematica code of SEIRQ Model for infectious diseases?

Or any book, paper

24 August 2023 6,459 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Paola Di Bonito

How many ul did you get? May be it is not a low yield

Omnia Usama

30-20 uL

Eliot Barson

Try freezing thawing at -80oC a few times after the addition of the TRI reagent to stop the degradation of RNA. Trizol has RNAse inhibitors.

You can be greedy whilst taking the initial aqueous phase if you repeat the chloroform step.

This way you can attain all the RNA and reduce the amount of impurities you take forward.