How to check normal distribution in a cell viability test?

To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...

02 February 2021 1,733 4 View

Best mounting medium for plant-tissue coloured with WGA-AF488 staining?

Hi everybody, I am preparing some plant tissue microscope slides, and I am doing a specific staining for fungi coloration (WGA-AF488 in PBS buffer pH 7.2). I was wondering what would have been...

26 November 2020 6,118 3 View

Why I observe different growth curves in Flp-In T-REx system?

Hi, I'm currently working with the Flp-In T-REx system to get isogenic stably transfected cells expressing my GOI (gene of interest, of about 1.4 Kb). I have generated a Flp-In Cell Line and since...

17 February 2020 5,608 2 View

Why does my MS show me only base peaks?

After changing the septum of my Focus GC connected to a DSQ II MS and after a complete cleaning operation on it, my mass spectrum became completely random. It shows me only base peaks in a...

10 June 2019 481 4 View

Can anyone suggest key literature and leading institutions in the field of Energy Transition?

Dear all, My background is in Energy Economics and Political Science and I would like to improve my knowledge on the Energy Transition issues. In your opinion, what are the most relevant and...

25 October 2018 8,507 7 View

How to simulate 3D beam to 3D beam contact in ansys APDL?

Hi, I'm modeling a lattice structure and I would like to insert the contact elements between 3D beams to get the whole structure non linear deformation. Since I never used contact elements before...

22 July 2018 1,551 2 View

GNSS Carrier phase Single difference residuals?

Dear all if I have the SD carrier-phase measurements how can I compute the SD residuals? Do i need to calculate the SD residuals before fixing the integer ambiguity?

10 April 2018 7,001 2 View

Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

15 January 2018 6,790 3 View

What is the main goal of modeling, and maybe of scientific knowledge tout court?

While ideally aiming at understanding an empirical phenomenon, and at the same time predicting its behaviour, scientists often focus only on one of these two aspects. Is this the dilemma of a...

13 November 2017 6,036 5 View

Are there physical applications of Banach-Tarski Paradox?

Banach-Tarski Theorem (also known as Paradox) is a mathematical statement which says that a sphere can be splitted into two (or an integer N...) spheres each equal to the original one. Do you know...

30 October 2017 9,335 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View