I am dealing with Candida RNA isolation from TRI reagent (used qiazen kit for extraction).I am really concerned about the quality of my RNA. According to Nanodrop reading my yields were ~100 to 150ng/ul. The A260/280s were ~2.1-2.2. Although I have used glass beads and vortexed vigorously, the quantity of RNA was not good. I am planning to use SAB (Sodium acetate buffer) for cell suspension and Phenol:Chloroform: isoamyl alcohol instead of TRI. Has anyone experienced on Candida RNA isolation without mechanical disruption? Please suggest me which one is better?

Thanks in advance