I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the same stock)
I give it to two Wistar rats that both weight 470g, IP 500ul, one rat anesthetizes without problem, but the other one rat fails to anesthetize and stay awake.
This is happening more and more often; I don’t know the reason or how to solve it.
Thank you.
The reason is difficult to elicit. Check the expiration date of your drugs. Bad storage and transfer of some drugs may affect the efficacy of some drugs {e.g. some drugs may be markedly affected if left in the sun outdoors during transfer from a factory or a distribution point}. Remember that IP drugs are injected in an invisible manner and may not be floating in the peritoneum, instead, it may be injected in a fatty collection or in the intestinal lumen.
To solve the problem try increasing the total volume of the injectant for proper distribution. Also try IM injection (470 gm is a big one) for proper dosing and efficacy. Remember that these drugs are made mainly for IM administration. Good Luck
Pre-anesthetic fasting is usually not necessary for rats due to their inability to vomit
Rat eyes remain open under anesthesia. This can lead to corneal drying and trauma
Though difficult to find reason on drug or animal, etc. I assume that Improper IP administration may be the reason. My suggestion is try proper IP administration.
Better to administer 5mg/kg xylazine HCl & 50 mg/kg ketamine intramuscularly@Kalaiselvan Elangovan
Better to administer 5mg/kg xylazine HCl & 50 mg/kg ketamine intramuscularly@Yunghao Tung
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