Our lab is trying to establish a BT-474 xenograft model. We have not been able to get the tumors to grow past 50-100mm3. We inject 1x107 cells in a 50:50 matrigel suspension. We used nude mice (5 weeks old) that have been implanted with estradiol tablets (90-day; 0.36mg). After 5 weeks the tumors have stopped growing and some are shrinking. Does anyone have experience with this model and can offer any advice? Should we go up on the estradiol dose? We used 0.36mg to avoid toxicity. Thank you!
Our lab has had succes with establishing the BT-474 xenografts (https://www.rug.nl/research/portal/files/48622056/Complete_thesis.pdf, chapter 4). What volume of cell suspension did you use and with which matrigel? The estradiol dose seems to be the same and has not been an issue in our hands.
BT474 are quite sensitive to the growth factor composition and grow rather slow, so I'm not surprised it would take longer than 5 weeks to fully develop. Have you monitored them longer than 5 weeks?
We have performed xenograft of BT-474 cell
https://www.mdpi.com/2072-6694/12/7/1811
This paper shows about intracranially injection.
In addition, we have experience of tail vein injection, intra-caudal artery injection and orthotopic transplantation of BT-474. But tumor growth of BT-474 cells are very slow. it takes 6 month for 300 mm^3 in orthotopic model with 10^6 cell injection.
BT-474 cells express ERBB2(HER2), so we did not use estradiol.
Thank you all for your suggestions! To answer some of the questions, we inject in the mammary fat pad with 50uL cells (1x107) and 50uL Corning Matrigel Matrix. We have been monitoring the mice for 6 weeks now, and will continue to monitor them - hopefully they are just slow growing as others have stated. However, some of the tumors have completely disappeared at this point. Thank you again for your suggestions and protocols!
Hi Jessica, just wondering if you have successfully built the BT474 xenograft? If so, could you share your experience? Thanks!
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