Hi

Please try sparse sampling method. For example for one time point you can take single sample from 1-3 different rats (rat number 1, 2, 3) for next point single sample from 3 next rats (rat number 4, 5, 6). You can reduce number of animals and leave them alive.

Article Sparse sampling approach in rodent toxicokinetic studies to ...

Article Serial Versus Sparse Sampling in Toxicokinetic Studies

https://academic.oup.com/ilarjournal/article/57/2/157/2806663

Examples how software can solve such set of data in NCA PK:

https://onlinehelp.certara.com/phoenix/8.2/topics/ncasparseexamp.htm

Example of study design:

https://www.psiweb.org/docs/default-source/default-document-library/tox_sig_2017-presentation-slides.pdf?sfvrsn=e0a5dfdb_0

I hope this helps you .... and your rats as well :-)

Best regards

Tomasz

Hi

Please try sparse sampling method. For example for one time point you can take single sample from 1-3 different rats (rat number 1, 2, 3) for next point single sample from 3 next rats (rat number 4, 5, 6). You can reduce number of animals and leave them alive.

Article Sparse sampling approach in rodent toxicokinetic studies to ...

Article Serial Versus Sparse Sampling in Toxicokinetic Studies

https://academic.oup.com/ilarjournal/article/57/2/157/2806663

Examples how software can solve such set of data in NCA PK:

https://onlinehelp.certara.com/phoenix/8.2/topics/ncasparseexamp.htm

Example of study design:

https://www.psiweb.org/docs/default-source/default-document-library/tox_sig_2017-presentation-slides.pdf?sfvrsn=e0a5dfdb_0

I hope this helps you .... and your rats as well :-)

Best regards

Tomasz

Ok , i got it ...

thank you very much

1- TAIL VEIN BLOOD COLLECTION .

2- SAPHENOUS VEIN BLOOD COLLECTION.

3- DORSAL PEDAL VEIN BLOOD SAMPLE COLLECTION .

4- ORBITAL SINUS BLOOD SAMPLE COLLECTION .

5- CARDIAC PUNCTURE .

Hi Zahraa

try this technique.

https://www.youtube.com/watch?v=yvqr1SHvPiI

Regards

For the pharmacokinetics study its need to collect the blood several times a day within time intervals. In my own experience, the Retro orbital (from the eye side) blood collection system is the best ever I think.

Why not take few samples and then apply deconvolution.

you can get more information from sparse sampling ideally by applying population modelling techniques but Phoenix also makes sparse NCA easier.

https://onlinehelp.certara.com/phoenix/8.2/topics/ncasparseexamp.htm