I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.
Xylene please.
For my PhD dissertation: If Chapter 1 is a systematic literature review (QUANTS/QUALS) while Chapters 2, 3, and 4 are empirical chapters (QUALS), is this considered a mixed methods research...
16 June 2024 3,220 5 View
I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.
05 June 2024 3,831 0 View
Hi, are there other methods beside Western Blotting to detect a specific protein? I‘ve isolated mitochondria and want to show that hPar17/Pin4 is present in mitochondria. The Western Blots didn’t...
24 May 2024 5,500 3 View
Good afternoon, everyone. Perhaps someone knows how many keratinocytes and fibroblasts are contained in 1 mm^2 of skin. Or the ratio of these cell types may be known. If there is a link to an...
15 May 2024 5,256 0 View
Hi! I´m currently trying to isolate Mitochondria from HCM cells. For my western blots I need an additional antibody against a mitochondrial protein which is bigger or smaller than 14/17 kDa. I was...
24 April 2024 8,814 2 View
Hi, I have having an issue with the Fc blocking stages when carrying out flow cytometry on a murine macrophage cell line. I am using a rat anti-mouse antibody against the scavenger receptor...
22 April 2024 3,466 2 View
i am currently conducting a critical appraisal of a research article and the data analysis is explained as: interviews transcribed verbatim and participants were given the option to review and...
21 April 2024 1,821 7 View
For a project I'm included in I'm desperately in need of audio recordings of male mice. They need to be original files recorded with the correct equipment. I need them for research on gait...
19 March 2024 4,959 1 View
lower year students attitude towards higher year students in America
09 March 2024 4,747 1 View
What is my research gate id?
29 November 2023 3,950 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
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24 July 2024 1,559 1 View
anti anaemic potentials if hibiscus sabdariffa
01 July 2024 8,429 0 View
Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...
01 July 2024 9,744 0 View
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
19 June 2024 5,950 1 View
Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...
16 June 2024 4,759 0 View