Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

How to converge hyperelastic modeling with large rotations in Abaqus?

Hi, My problem is similar to simulate fold soks in 3D model with 2D elements (shell). I would like to use Membrane element but it is not possible for hyperelastic material in abaqus. I use S3 (or...

17 March 2020 795 5 View

How to properly calculate the mutational rate in bacteria?

Hello everyone, I hope y'all can answer a simple but problematic question. I have been doing mutation accumulation experiment in 3 parallel lineages in bacteria and I now want to calculate the...

05 December 2019 5,648 4 View

Does anyone know how to implement restricted permutations for multivariate analysis of split split plot experiments in R?

02 April 2019 4,257 1 View

Is there any effective transfection protocol for cells cultured in 3D hydrogels?

I would like to transfect micro-RNA mimics in 3D models. Lipofectamine RNAimax works well in monolayer cultures but I couldn't find convincing evidence it could work sufficiently well in 3D.

27 November 2018 4,524 3 View

How to design a low ecological footprint electric machine?

To my knowledge, electric machines (motors and generators), apart from their electronics, are made of at least two or more of these materials: Electric steel (stator and rotor iron...

08 November 2018 1,783 9 View

Quickly pellet floating fatty cells in culture plate?

Hi, I have fatty floating cells in culture medium and I need to simply/quickly pellet them without degrading cells nor removing themfrom the plate. Any idea ? thanks

21 March 2018 4,569 3 View

Suggestions for an undergraduate research project concerning Bryophytes and Plant Soil Feedback?

I'm planning my Undergraduate Research Project and am very interested in the field of Bryology (I'm majoring in Botany), and Plant Soil Feedback, but am having a tough time coming up with a...

16 January 2018 9,890 4 View

Help with NCBI table2asn

Does anyone have experience with the table2asn tool of NCBI? It is used for submitting annotations of genomes using GFF files. It can be found at this location:...

23 May 2017 1,696 3 View

What is the easiest way to determine the absorption spectrum of a bacterium?

We would like to determine the absortion spectrum of a bacterium. I have, however, never done something like that. It's to see if there are any absorption peaks present and if the bacterium would...

05 January 2017 6,933 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View