When the rats were induced diabetes with the administration of Streptozotocin Section from pancreatic islets and acini shows cytoplasmic vacuolation. Islets are small in size with reduced in the number. When the rats were given the standard Glibenclamide drug, Section from pancreas shows pancreatic acini with mild focal cytoplasmic vacuolation. Islets are reduced in number with few showing small in size. When the rats were given 200 mg extract
Section from pancreas shows normal pancreatic acini. Islets are normal in number with small in size and focal cytoplasmic vacuolation.. When the rats were given 400 mg of extract
Section from pancreas shows normal acini. Islets are normal in size and number with cytoplasmic vacuolation. Blood vessels show congestion. Please explain to me.
For a histopathologist the most important thing is the nucleus and not the cytoplasm. The nucleus will tell you about necrosis, apoptosis, cell death etc. You need to look for signs of necrosis such as karyolysis, kariorrhexis or pyknosis which indicates irreversible damage. The cytoplasmic vacuolation just indicates that the cell is under distress and most of it reversible.
You need to get a bit better histology sections. Your STZ does might be a bit higher or you can try using an antioxidant along with STZ in order to minimize the damage.
Histopathology is a tough subject to study. Remember you first need an MBBS and then 4-5 years of training before they will certify you as a competent histopathologist. If you want to learn more on histopathology you can study the following website:
http://www.pathguy.com/histo/000.html
He has 100 slides and some of it describes the islets of Langerhans.
Regards
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