How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

How to characterize the dispersion of aluminum oxide in Al matrix composite which reinforced by alumina during the powder metallurgy?

Now we could characterize the disperion of Al2O3 in AMCs (pure Al reinforced by Al2O3 ) after sintering. But the process of powder metallurgy has a lot of steps, which take long time. The cycle...

08 November 2020 5,244 3 View

Is that possible the filter of flow cytometry was failed so I got those data using FL1 vs FL2 and FL1 vs FL3?

The cells were expressing GFP and also incubated with PI for dead cells detection. This is my first-time using this BD Accuri C6 and I'm not sure if I need to set compensation (I tried to follow...

01 November 2020 8,360 3 View

Different Ct values from repeat standard curve preparations and re-using same primers for different DNA containing same target region for qPCR?

Hello I've performed qPCR experiments for many years but I could not really figure out the following. First question: I've prepared standard curves from a DNA template (concentration determined...

20 August 2020 8,785 4 View

What if the abbreviations of two technical terms in the manuscript are the same?

I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?

23 June 2020 2,115 5 View

ImzMLConverter installation & running issues?

Hi Everyone, Did anyone have a similar issue when install and run imzMLConverter? I followed the instruction on https://www.cs.bham.ac.uk/~ibs/imzMLConverter/ and used the command it provides....

23 May 2020 3,936 3 View

How to revise survey items based on unexpected EFA results?

Hi, there. A few days ago, I posed a question about factor analysis of survey results, but I left many important details unspecified. HERE is the more detailed one. I develop a theoretical model...

06 May 2020 4,937 10 View

Many cases of identical answers to consectutive questions.How to revise survey items?

Hi, there. I am working on my Ph.D dissertation. I have run a factor analysis of 56 items with maximum likelihood and direct oblimin method. It turns out that only one factor can be extracted...

25 April 2020 6,267 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View