Which is the best approach for nucleotide substitution model-testing for phylogenetic reconstructions using RADseq data?

I am trying to infer both maximum likelihood and bayesian phylogenetic trees using RADseq reads. I have already created my sets of radtags using Ipyrad which also I used to export the consesus...

24 August 2020 7,823 8 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

How to separate over expressed protein?

I have to identify the protein responsible for some activity in microbes and have seen over expressed band in native page. how can i purify it for protein sequencing.

21 October 2019 3,626 2 View

Practical way to model moment free joints in ANSYS?

I am designing a complex roof structure with hundreds of moment free joints. I have found that using the end release option in ANSYS I can model this behavior. The limitation is that ANSYS only...

25 September 2017 4,663 8 View

What is the height of cancer cells?

What is the height of cancer cells growing in 2D cultures? I am mostly interested in human pancreatic BxPC-3 and murine breast cancer 4T1 cells.

22 September 2017 1,981 2 View

How to show that ultrasound exists in the cel medium

We are applying ultrasound for live cells grown in multi-well dishes. There is no a noticeable effect on cell viability. How to show that there is an effect of ultrasound at all in the cell medium?

20 July 2017 3,415 2 View

Does anyone know about ethnographic or historic references to the use of inebriants among any of the African societies mentioned below?

20 December 2015 9,370 5 View

What is the best method for measuring endothelium function?

See above

21 September 2014 360 20 View

Does anyone have any experience with measuring impedance of powder samples (without sintering ceramics)?

09 July 2013 5,009 9 View

What are the optimal conditions to culture Kupffer cells?

I am currently doing Kupffer Cell primary cultures (by differential centrifugation). What are the optimal conditions to culture them? I currently use standard culture-treated 24 well plates and...

31 October 2012 3,252 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View