I am currently trying to perform co-culture of Astrocyte/Schwann cell (primary culture), for Immunostaining I have to use antibodies specifically recognize each cell type, to do so I have been using GFAP/P75/S100. the antibodies work well for monoculture but when it comes to coculture although the two primary antibodies from differenet species and each secondary antibody has different fluorochrome but I cannot visualize both types of cells. as i got aware right now GFAP not only specific for astrocyte but could also stain schwann cell, so which marker is best and specific for each astrocyte and schwann cells?
I would also need to look at their morphology in deeper context when culturing together
Any advice and suggestions would be highly appreciated. Thx
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