the only method to determine toxicty by following the biochemical parameters of liver, kidney m brain ,, etc.. However, use the same ratio for adults

Thank you sir. As per toxicity studies the dose of ethanol extract for adult rats is 600mg/kg. Can we take same dose for rat pups.

Check https://www.mdpi.com/2077-0383/9/4/1176/pdf

Check https://www.science.gov/topicpages/a/acorus+calamus+extract

Thank you sir for sharing the link. it was useful

Hello, thank you for writing to me, you could do it by determining the toxicity with an Oral Toxicity test to the extract, and after the sacrifice of the animal, support yourself in a histological study with a pathologist.