After contextual fear conditioning + 4-OHT injection I can see dtTomato-positive cells in several regions of the brain (mice are sacrificed 4 days later) but nothing in the dorsal CA1. Can someone explain to me why ? Thanks a lot
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...
22 May 2024 1,763 0 View
I am a master student at Erasmus university and writing my thesis about branding in the banking sector. I am desperately looking for participants for my interviews, a lot of companies are too busy...
02 April 2024 3,264 1 View
Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...
20 December 2023 7,869 0 View
I still don't figure it out yet how exactly a virus particle is formed by adding three plasmids (transfer plasmid with transgene, packaging plasmid and envelope plasmid) in a cell by...
27 November 2023 5,725 4 View
My lab is experiencing a new issue with several mouse brain tissue sections (for confirming placement, no immunohistochemistry). Each tissue section has white "sparkles" that dissipate over time...
12 November 2023 1,757 0 View
The primary objective of this inquiry is to establish the existence of a validated Medical Student Self-Efficacy Scale in the French language. This question is motivated by the significance of...
31 October 2023 5,676 0 View
I am having trouble getting consistente results with MAIPS4510 ELISPOT plates (Merck, Millipore). We have used them before with no trouble and using the same protocol. Or positive controls (PHA)...
26 October 2023 7,248 0 View
Dear all, I'm looking for an alternative for a CO2 incubator with O2 regulation as these are quite expensive and I actually just need 5% O2, 5% CO2, 37°C over 3 days every 2 weeks (should fit...
10 April 2023 5,395 2 View
Hi, I'm working on a research proposal for a pilot study and struggling to land on the optimal sample size. The quant measures (physiological measures and surveys) are recorded while the...
27 March 2023 9,408 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View