Hello there. I just completed an experiment using both hM4D(Gi) and hM4D(Gq) DREADDs in the cerebellar vermis, both co-expressed with mCherry. I noticed that while I had a lot of expression of the Gi DREADD in my histology, there was little to no expression of the Gq. Has anyone else observed this/ seen articles where this may be the case? Thank you!
Activation of hM4D reduces membrane excitability. Several Studies had been made regarding its expression in hippocampus which is the temporal extension of cerebrum. The selective expression of hMRD(Gi)_in principal hippocampal neurons have seizure suppressing effects. Categorical quantitative expression of it in cerebellum had not been studied. So comparative conclusion may not be possible. Might be, its expression in hippocampus is very important.
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