Does anyone have any interesting articles on goal setting and it's use in physiotherapy?

I am currently a MSc Physiotherapy student at Teesside university and I have decided to look at Physiotherapy Students Perception on Goal Setting. It would be useful if anyone had useful articles...

13 February 2019 6,266 6 View

Cloning: Best method to remove two 1kbp sections from a plasmid?

Hi Everyone, I find myself in the awkward situation of having repeated rounds of PCR fail with a rather large and GC rich gene. I've order the gene to be synthesized in a codon optimized form onto...

30 August 2017 121 4 View

If you have transformed data for statistical analysis when you come to publish do you present the original or transformed data?

I did some lab experiments and because of huge variance I needed to log and square root transform the data to be able to proceed. I'm now planning to submit for publication but I can't recall...

06 July 2017 4,980 6 View

Has anyone developed a Batesonian 'methodology' for investigation?

I work in Organisational Development using a mix of systemic and dialogical principles and want to bring some rigour (and imagination) into my use of the ideas of Gregory Bateson and wondered...

07 June 2015 7,071 4 View

How can I convert genome-wide protein alignments to codon alignments?

Given a large set of protein alignments, and having the corresponding DNA sequences, I would like to find a high-throughput tool to generate the codon-based DNA alignments of these sequences. By...

29 September 2013 1,206 5 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View

Is it possible to find the radiative/nonradiative lifetime without steady-state conditions?

I have a set of time-resolved PL measurements, to which I applied an exponential fit to extract the effective decay time. I now want to determine the radiative and nonradiative rates involved in...

03 February 2021 7,674 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View