In my laboratory there are many primary antibodies wich were bought in 2014. we want to know if these antibodies are still available for our immunoblots.
Does anybody knows about it?
We aim to detect p62 protein expression levels. Previously, we worked with the p62 antibody (Cat. No: A19700, dilution 1:1,000, ABclonal) and obtained excellent results. However, we switched to a...
20 June 2024 327 3 View
So I am doing some physiology on acutely prepared brain sections (350um) and want to preform IF on these slices following treatment + response. The one tried and true method is doing an extended...
28 May 2024 9,859 0 View
Someone could help me? When I scan my PVDF membrane with a LI-COR scan after immunnoblot it looks dark. Someone knows what could be wrong in my western blot?
26 January 2023 239 8 View
I have been looking for ViBo cervical cancer cells information in the ATCC but I can´t find nothing about it. I ask for help if anypeople know about an article or web site for consult ViBo cells...
10 January 2023 7,467 3 View
For your information, I am using ABI StepOne Plus machine. The housekeeping gene is Beta-actin. However, the results showed its melting curve and fluorescence plot look good. Appreciate it if...
13 November 2022 9,862 1 View
Hello, I need to be able to measure approximately 1mg/ ml of dissolved PHMB. I have considered complexing the PHMB to Iron and then using HPLC with a spectrophotometer. Or using LC-MS. Is...
05 September 2022 2,259 0 View
If the use of design thinking could be useful in concept and incident idea generator that could find those which start the ball rolling.
31 March 2020 2,505 7 View
I am currently a MSc Physiotherapy student at Teesside university and I have decided to look at Physiotherapy Students Perception on Goal Setting. It would be useful if anyone had useful articles...
13 February 2019 6,326 6 View
Hello All. Am seeking any literature that explores: - adsorption rate of long-chain PFASs out of water/s onto solids (esp. built/manufactured structures such as concrete, steels, plastics etc)...
12 December 2017 8,962 0 View
Hi Everyone, I find myself in the awkward situation of having repeated rounds of PCR fail with a rather large and GC rich gene. I've order the gene to be synthesized in a codon optimized form onto...
30 August 2017 177 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View