Please, how can I sterilize a nano preparation if it is affected by light, and it is in colloidal state and may lock the syringe filter? Later I have to study its effect on cultured cells.
Thanks in advance
Ruling out filtration by 0.22 um since you say that the nanoparticles clog the filter, if your nanoparticles can withstand an autoclaving cycle (120 ºC) this would be the most economical option. Another option is gamma radiation. If your nanoparticles are affected by temperature and radiation (as is common), you should produce them in aseptic conditions to maintain their sterility. Here you can see a paper in which the effect of nanoparticles on cells in culture was studied, where you can see the established protocol:
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