What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Disappearing Coomassie Bands?

Suddenly my Coomassie staining has produced strange results (see photo attached). The past 8 gels all share the same trends: the bottom half of the gel fades out and is absent of any bands. I...

19 December 2020 3,619 1 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

NMR-SIM not installed on TopSpin 3.6.2?

I have recently installed TopSpin 3.6.2 in a Windows 10 where I had already TopSpin 3.6.1 working correctly. After several attempts in installing and uninstalling I am still unable to use NMR-SIM....

18 November 2020 1,688 2 View

Masters thesis subject - International humanitarian law/ International criminal law?

Hello I am a master's student and I'm searching for a current debate in the field of international humanitarian law or international criminal law. Is there somebody who can help me with this?...

08 November 2020 1,571 13 View

Free database for auditory stimuli?

Hi, I'm currently looking for auditory stimuli (list of letters; word/non-words; etc) to include as distraction task in my experiment. Does anybody know a free database that I might download?...

04 October 2020 9,614 3 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Can anyone tell me which species (or higher taxon) this is?

We found this small, maybe parasitic, crustacean in the North Sea offshore. The posterior body part is missing, but it looks quite characteristic. There is double-hooked rostrum, 2 antennae...

08 April 2020 6,842 9 View

Can I use time as a predictor variable in a panel regression?

Is it possible to generate a new variable "phase" that is based on the time the participants were questioned? I generated this new variable by dividing the time into three phases (beginning,...

30 March 2020 3,756 6 View

Dielectric constants of organic:aqueous mixtures, is linear addition a valid approximation?

I would like to compute dielectric constants of organic:aqueous mixtures. Would linear addition of the dielectric constants of the pure solvents give reliable results? For example, for a volume...

26 March 2020 5,939 3 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View