Hello,
I want to work with some gene expression data that is recorded in TPM. The data has two biological replicates with their own TPM values. How should I go about using this data? is there a way to average the two TPM values so I can just have one value that is representative of the expression levels?
Thank you
The link below may help:
www.docs.microsoft.com/en-us/windows/security/information-protection/tpm/trusted-platform-module-overview
Hi Clarissa,
I assume you need this to get an overview over your data and/or calculate fold changes between conditions. It is possible to average the TPM values in this case. Be careful when performing statistical analyses on these data though, you should use a proper tool that adjusts for multiple comparisons.
Hope this helps.
Best regards,
Alexander
I feel the environmental conditions may be different for two different genes like G*E effect the people talk about. As far as statistics is concerned, pls take 2 sample t - test and find whether there is a difference in the samples or no. If not then u can simply add provided domain knowledge allows. generally log distribution is used for biological analysis so pls study carefully as numbers are in millions of millions...
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