These are initially solitary rod like small structures floating in the media, and then they start forming aggregates. Could this be bacillus? How to deal with it.
These look like crystal precipitate in the media, not a biological contaminant. Depends on the media but probably look like some salt or like calcium oxalate crystals
Hi Yashvi Sharma
I agree with Antonio Hrvat - depending on the size, you could possibly filter you media to remove impurities.
Incubate culture plate with media and no cells. Check for these crystals. Seems like they might be ascorbic acid crystals. Change in pH (due to metabolic activities of cells in culture) may also result in crystal formation. Check pH of your culture (with and without cells), and also check % of CO2 in the incubator.
(https://www.researchgate.net/post/How-do-I-prevent-the-formation-of-crystals-in-cell-culture)
Good luck!
Antonio Hrvat Jack Hopkins Savita Visal-Shah
Thankyou for your suggestions, we check the media before using for cell culture, but they crystals do not form there, and these go on increasing in numbers and are initially solitary but then start forming aggregates, also i have seen them being stuck upon cells and not hampering their growth.
If they are floating, and your cell line is adherent, you should be able to remove by washing your cells once or twice in PBS before changing media.
Have you tried to spin it (centrifuge)? if they still floating then it could be a product like cellulose.
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