Hello,

My current lab is analyzing qPCR data in a method that is frankly, very incorrect. Can anyone recommend a step-by-step example for analysis of significant differences between treatments, and generating an appropriate boxplot of the data?

Our experimental setup measures one target gene and two reference genes. There is an untreated control group, and two or more treatment concentrations. I want to be able to test for any significant effects of the treatment, and generate a boxplot or dot plot to show the results.

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