Does anyone have insight on the isolation and cell culture of Type I Alveolar Epithelial Cells?

10 January 2021 0 834 Report

I am looking for isolation (FACS) and cell culture protocols for rat Type I alveolar epithelial cells. I have read "Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs" by Drs. Gonzalez and Dobbs and wanted to know if anyone has used this and what your experience has been.

I would love to hear from anyone that has experience with isolating and culturing Type I alveolar cells - any advice, insight, or tips would be appreciated!

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