I am wondering which are the antibody commercially available to detect specifically the proteasome subunits in budding yeast (Saccharomyces cerevisiae). Any suggestions and recommendations really appreciated.
Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.
23 February 2021 9,086 3 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
22 February 2021 215 2 View
As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?
19 February 2021 5,509 3 View
I have remotely sensed data (image) that I want to use in the Neuro-Fuzzy Designer apps of MATLAB. Is it possible to use an image file in the Neuro-Fuzzy Designer app or only numeric data is...
17 February 2021 1,205 5 View
To determine % K2O of organic manures, flame photometric technique is used whereas in inrganic fertiliser for K2O determination STPB method is used. I am trying to find out any method of...
24 January 2021 4,714 3 View
Hello, I had been trying to model a portal frame wrapped with CFRP in ABAQUS. To tie the CFRP laminates with frame I need to use slave/master surface interaction. But whenever I try to model the...
19 January 2021 2,906 3 View
I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...
18 January 2021 3,578 1 View
These days I am noticing that the mixture of NaOH (NH) (10-12M) and N2SiO3 (NS) solution gets solidify after 24 hours. The ratio of mixture of NS to NH is 2. To mention, the same solutions that we...
18 January 2021 7,911 2 View
Respected sir/mam, I want to simulate the urban expansion using different time series LULC based on satellite image. please suggest me most suitable model for urban simulation. Thanks and regards.
17 January 2021 9,134 11 View
Hello, Can you help me regarding MATLAB solver for a system of stochastic delay differential equations? To be specific the system is delay differential equation where parametric stochasticity is...
15 January 2021 6,950 7 View
I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...
28 February 2021 298 3 View
One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...
21 February 2021 418 2 View
Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...
16 February 2021 9,050 5 View
In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...
15 February 2021 8,018 2 View
I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...
05 February 2021 6,379 5 View
Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...
25 January 2021 4,228 6 View
I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?
25 January 2021 958 4 View
We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...
24 January 2021 9,830 3 View
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...
22 January 2021 585 10 View
I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...
18 January 2021 1,023 3 View