Proteins

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Questions related to Proteins

Can I use vdW correction in LDA calculation?

04 June 2022 8,939 0 View

How can I calculate the volume of a voronoi polyhedra? Can I calculate using Ovito?

I want to calculate the volume of Voronoi Polyhedra so that I can calculate effective free volume. I have no idea how to calculate the volume of Voronoi Polyhedra. I have tried using ovito but I...

29 May 2022 9,879 0 View

Does anyone have a protocol for biotinylating an AviTagged protein in HEK cell lysates?

Yes, we could just express BirA from a plasmid, but we hope to eventually use this protocol in cells that are difficult to transfect. It would be more convenient to just toss some BirA into the...

06 April 2022 9,060 1 View

What is the isotopic discrimination between bone collagen and muscle?

Dear all, I am looking for TDF between muscle and bone collagen in ungulates/herbivores to recalculate C13 values of collagen to muscle values. Best, Maciej

14 January 2022 2,837 0 View

Is it normal for a compound to possess more than one CCS?

I was evaluating the collision cross section (CCS) values for various compounds using traveling wave ion mobility spectrometry (TWIMS). Quite beyond my expectation, some compounds seem to possess...

31 December 2021 6,624 10 View

Does DMSO shows any signal in CD spectra?

Hello- I would like to record the CD spectra of short peptides dissolved in DMSO. When I measure DMSO alone as a baseline, there are some intense signals between 190 and 250 nm. How can I resolve...

29 December 2021 9,674 3 View

Can anyone provide a FID chromatogram or elution order of the following fatty acids?

I'm looking for the elution order of the following fatty acids in C18:1n9t, C18:1n9c, C18:1n7, C24:1, C22:4n6, C22:5n3, C22:5n6, and C22:6n3. I use Supelco SP 2560 Capillary GC Column.

02 December 2021 1,244 3 View

Biomarkers for AD?

I am working on looking for a biomarker for Alzahimer diseases. When I compared the results for treatment cells to basal in AD group and treated cells from healthy group vs basal, I see a...

28 November 2021 4,534 4 View

What are the recommended software for Voronoi diagram analysis in a image ?

I want to study the defectivity of hexagonally packed cylinders. I prefer to use methods like Voronoi diagram analysis or Delaunay triangulation analysis for the defect analysis in the SEM and...

23 November 2021 4,643 1 View

Can arabinose inducible promoter be engineered in yeast?

does someone know a paper where authors engineered the arabinose induction system of prokaryotic organisms in yeast Saccharomyces cerevisiae, like an IPTG induction system, for orthogonal gene...

04 November 2021 7,838 3 View

Is it possible to convert mg/L to kg/ha?

I am gathering data from papers and some authors present the availability of nutrients as kg/ha and others as mg/L. Would that be possible to convert mg/L to kg/ha once L is volume and ha is...

27 October 2021 987 14 View

Do we calculate Athematic Mean for Gender?

I have seen most of the Research papers will calculate Mean for Gender as Gender is Categorical variable only we can calculate percentage as it comes under Nominal scale the following article is...

20 September 2021 716 4 View

Detection of soluble recombinant protein aggregates by native PAGE?

I have been able to express a soluble 6xHis-tagged protein but purification is extremely inefficient. We are only able to increase our yield by overloading the resin (8-10 ml lysate on 50 ul...

13 September 2021 5,964 3 View

How to remove methionine at the N-terminus of Ovalbumine?

Hello, I ran into a serious problem during my experiments. For some reasons, I need glycine at the N-terminus of my ovalbumin (mammalian cell expression, slightly modified protein, ordered from...

08 September 2021 1,602 3 View

Mcf 7 cells not growing after 48 hours?

After fumigation of co 2 incubator, I thawed the cell line from where I used to thaw before too and after 48 hours media color changed to yellow and turbid. I used rpmi media from another lab and...

23 August 2021 1,041 5 View

Why am I not getting any plaques while doing top agar method?

When I spot the phage lysate on MG1655, I see the clearance. But, to calculate the phage titer I mix the lysate (100ul) with MG1655 as background strain (100ul) in 3ml 0.5% agar. In this case, I...

17 August 2021 7,202 21 View

Why does the band gap of PbO (1.9eV) and SnO (0.7eV) vary so much?

PbO and SnO have the same crystal structure and similar ionicity. Can it be explained by orbital molecular theory?

09 August 2021 469 5 View

When making selection media for a transfection, do I put the antibiotic in media with FBS and PenStrep or fresh media (without any FBS and Penstrep)?

I am working with HepG2 cells and transfecting them with a plasmid that upregulates the GABRA1 gene. I need to put them in selection media before I observe the results. HepG2 cells are resistant...

08 July 2021 2,969 2 View

What is the reason for non-standard DSC baseline shape?

Greetings! We use thermogravimetric analyzer TGA/DSC 3+ (Mettler Toledo) to study clay minerals, soils and other objects. Every single DSC curve we get (including empty alumina crucible, alumina...

05 July 2021 7,453 8 View

Can acrylonitrile butadiene (33/67) copolymer rubber block be converted into a creamy emulsion?

I ordered acrylonitrile butadiene (33/67) copolymer emulsion so I can do coagulation and dipping and form a nitrile product on a mould. I was expecting a creamy emulsion to arrive; however, I...

10 June 2021 8,004 2 View

Why women who employ a combination of breast along with bottle feeding had higher rates of PPD as compared to those who utilized only bottle feeding?

Ystrom found that, at six months postpartum, both partially breastfeeding and exclusively bottle-feeding were significantly related to higher levels of depressive symptoms in postpartum women...

21 April 2021 4,640 3 View

Did anybody observe large "multinuclear cells" in WT CHO cultures?

In our WT CHO cell cultures we often observe large multinuclear features looking like syncytium of some 10-20 cells fused together. What could it be? Would subcloning help to get rid of those...

21 February 2021 137 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

05 February 2021 4,756 4 View

Can you perform separate generalized estimating equations analysis?

In my current research I've done the following: A GEE analysis was run to explore significant predictors associated with severe postpartum depression symptoms across the 6-month postpartum...

25 January 2021 3,515 2 View

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