Proteins

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Questions related to Proteins

How to get a co-author properly associated with my research paper?

One of my publications appeared online first with an error in one of the authors name. The journal made the correction, but the RG website did not. How can we fix it?

12 February 2023 7,144 2 View

How to calculate photoelectron Circular dichroism (PECD) using quantum chemistry?

is it possible to calculate photoelectron Circular dichroism (PECD) using quantum chemistry?

10 January 2023 1,786 4 View

How to study the gradient of concentration of secreted factors between cells?

I am working on cell-cell communication in GBM cancer, and I would like to study the gradient concentration of some factors, specifically I want to detect that gradient formation between cells....

30 November 2022 9,330 0 View

I want to isolate autophagosome from sucrose/ ficol density gradient method? . can anyone provide me detailed protocol ?

Particularly for human brain tissue

29 November 2022 8,049 0 View

I want to do amino acid analysis in the seed meal of cotton. Can anybody suggest me the best method?

29 November 2022 4,679 0 View

Where can I get information about growth kinetics of Actinomycetes ?

growth kinetics of Actinomycetes?

25 November 2022 9,341 1 View

How to stimulate clonal growth of fibroblasts after single-cell sorting?

My protocol requires the establishment of clonal cell lines through the expansion of single cell clones sorted in individual wells by FACS. This is notoriously challenging for fibroblast cell...

24 October 2022 8,637 2 View

Does anyone know what is wrong with Vienna-PTM web server (http://vienna-ptm.univie.ac.at)?

Vienna-PTM web server (http://vienna-ptm.univie.ac.at): a toolkit for MD simulations of protein post-translational modifications.

03 October 2022 3,333 0 View

Half-life for protein biomarkers?

I am doing a study on how a certain exposure may affect (change) biomarker profiles in human serum, i.e. at baseline compared to 1 hour after the exposure. In order to detect any change, I assume...

27 September 2022 6,581 4 View

If a cow eats genetically modified grass will the meat and milk of cow deviates from its origin?

Outcome depends on the type of food it takes right ? So if a cow consumes Genetically modified grass rather than normal will the quality of milk increase? And can we observe any changes in the...

28 August 2022 3,807 3 View

Can recovery percent value be more than 100?

Recovery analysis is done for the evaluation of sensors as a ratio of estimated concentration and spiked concentration, expresses in percentage. I have came across many articles mentioning...

23 June 2022 4,984 0 View

Can I use vdW correction in LDA calculation?

04 June 2022 9,058 0 View

How can I calculate the volume of a voronoi polyhedra? Can I calculate using Ovito?

I want to calculate the volume of Voronoi Polyhedra so that I can calculate effective free volume. I have no idea how to calculate the volume of Voronoi Polyhedra. I have tried using ovito but I...

29 May 2022 10,021 0 View

Does anyone have a protocol for biotinylating an AviTagged protein in HEK cell lysates?

Yes, we could just express BirA from a plasmid, but we hope to eventually use this protocol in cells that are difficult to transfect. It would be more convenient to just toss some BirA into the...

06 April 2022 9,184 1 View

What is the isotopic discrimination between bone collagen and muscle?

Dear all, I am looking for TDF between muscle and bone collagen in ungulates/herbivores to recalculate C13 values of collagen to muscle values. Best, Maciej

14 January 2022 2,942 0 View

Is it normal for a compound to possess more than one CCS?

I was evaluating the collision cross section (CCS) values for various compounds using traveling wave ion mobility spectrometry (TWIMS). Quite beyond my expectation, some compounds seem to possess...

31 December 2021 6,766 10 View

Does DMSO shows any signal in CD spectra?

Hello- I would like to record the CD spectra of short peptides dissolved in DMSO. When I measure DMSO alone as a baseline, there are some intense signals between 190 and 250 nm. How can I resolve...

29 December 2021 9,821 3 View

Can anyone provide a FID chromatogram or elution order of the following fatty acids?

I'm looking for the elution order of the following fatty acids in C18:1n9t, C18:1n9c, C18:1n7, C24:1, C22:4n6, C22:5n3, C22:5n6, and C22:6n3. I use Supelco SP 2560 Capillary GC Column.

02 December 2021 1,364 3 View

Biomarkers for AD?

I am working on looking for a biomarker for Alzahimer diseases. When I compared the results for treatment cells to basal in AD group and treated cells from healthy group vs basal, I see a...

28 November 2021 4,687 4 View

What are the recommended software for Voronoi diagram analysis in a image ?

I want to study the defectivity of hexagonally packed cylinders. I prefer to use methods like Voronoi diagram analysis or Delaunay triangulation analysis for the defect analysis in the SEM and...

23 November 2021 4,770 1 View

Can arabinose inducible promoter be engineered in yeast?

does someone know a paper where authors engineered the arabinose induction system of prokaryotic organisms in yeast Saccharomyces cerevisiae, like an IPTG induction system, for orthogonal gene...

04 November 2021 8,013 3 View

Is it possible to convert mg/L to kg/ha?

I am gathering data from papers and some authors present the availability of nutrients as kg/ha and others as mg/L. Would that be possible to convert mg/L to kg/ha once L is volume and ha is...

27 October 2021 1,195 14 View

Do we calculate Athematic Mean for Gender?

I have seen most of the Research papers will calculate Mean for Gender as Gender is Categorical variable only we can calculate percentage as it comes under Nominal scale the following article is...

20 September 2021 827 4 View

Detection of soluble recombinant protein aggregates by native PAGE?

I have been able to express a soluble 6xHis-tagged protein but purification is extremely inefficient. We are only able to increase our yield by overloading the resin (8-10 ml lysate on 50 ul...

13 September 2021 6,071 3 View

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