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Questions related to Proteins
to detect change in milk after treatment by thermal method
06 June 2017 9,469 9 View
I believe it has to do with protein size, or perhaps it may be due to the fact that introns (which are needed for interleukin production) are much more rare in bacterial genes than in eukaryotic...
06 June 2017 8,779 0 View
I have many secreted factors for which I want to identify corresponding receptors and where these receptors are expressed.
06 June 2017 413 2 View
Hi Did anyone know what percentage of dry weight the portunus pelagicus crab shells was formed by protein and chitin?
06 June 2017 2,636 2 View
Does it means two proteins are similar
06 June 2017 5,814 3 View
I'm working with a protein which don't have any metal in its structure. In in-silico study i want to show how metal is binding at the catalytic active site of my protein during reaction as metal...
06 June 2017 3,704 3 View
I have been analyzing different online servers in predicting Bcell epitope for a protein. But each server giving different result i.e displaying epitopes at different positions. So I couldn't come...
06 June 2017 7,115 3 View
We have been seeing a change in the electrophoretic migration of a cellulose-active protein after being incubated with its substrate. Does someone know what could be happening? Could it be that...
06 June 2017 3,471 7 View
What is the simplest, benchtop way, to find out the proportion of a drug bound to protein in the brain following systemic treatment in vivo?
06 June 2017 6,675 3 View
I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...
06 June 2017 4,258 4 View
I am trying to characterize a so far unknown membrane protein (a GPCR). I overexpressed it in HEK cells and want to verify its abundance via WB. So far there is one problem: WB analysis shows...
06 June 2017 7,429 3 View
I have used patchdock for docking two protein subunits. I have got the top 10 or 20 results from these servers. Can anyone please suggest how do i determine or evaluate the best out of the given...
06 June 2017 2,395 2 View
Hello friends, During proteomic analysis of stem cell secretome, we observed that secretome is significantly enriched for PFAM protein domain (kinesin motor domain). I was also wondering about...
06 June 2017 3,052 3 View
04 June 2017 3,823 3 View
02 June 2017 7,040 3 View
01 June 2017 7,682 2 View
Dear all I have performed ITC for protein and ligand solution.generally we substract heat of dilution of ligand from overall heat for ligand titration into HSA .is it necessary to substract the...
31 May 2017 4,375 4 View
Hello everybody! I always get so good answers and help, so I'll ask you experts again. I use rat cardiac fibroblasts and do ELISA to measure cGMP. For lysis, the protocol says one can use either...
31 May 2017 1,204 3 View
Hi. Does phosphorothioate CpG ODN remain unaffected by digestive tract environment in poultry, when used as oral adjuvant? What is the minimum/optimum amount of CpG ODN suitable to be used as oral...
30 May 2017 3,685 19 View
Good morning everybody. I am purify MsrA from E. coli. I first run an anion exchange and now I am running a Phenyl sepharose (HIC). the buffer I am using is 20mM MOPS pH7 1.2M Ammonium sulphate...
30 May 2017 7,912 5 View
I am trying to make a ketogenic diet using lard and wonder how to dissolve lard and have a solidify mix of carbohydrate+protein+ lard at the end for 1 litre?
30 May 2017 7,206 3 View
I have a protocol where they used protein G sepharose beads with another tag, but I'm not sure that protein G sepharose is compatible with the sigma flag antibody, or any other flag antibody.
26 May 2017 9,076 3 View
Hi everyone I have problem for quantify exosome protein with BCA, however using Qubit assay I have measured, but I don’t know if this protein concentration is good for my work. My question is...
25 May 2017 3,861 3 View
I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...
25 May 2017 1,019 3 View