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Questions related to Proteins
Hi there I have been doing microplate bradford assays on protein samples (they are relatively crude). I have used BSA as a std and each time the standard curve is consistent in slope and...
03 March 2018 4,084 5 View
I have a 1mg liofilized stock of a membrane protein prepared in PBS, but for my next experiments i'll need the protein to be prepared in other buffer. How could I change the buffer without loosing...
03 March 2018 6,375 4 View
Why do protocols use TFA to stop the guanidination reaction?
03 March 2018 9,777 7 View
In all literature I read the cyanylating agent 1-cyano-4-dimethylaminopryidinium tetrafluoroborate (CDAP) is used for cross-linking to sugars, in these we obviously have our hydroxyl oxygen...
03 March 2018 779 2 View
I have green micro-algae (Enteromorpha) powder and want to measure the carbohydrates and proteins before and after the anaerobic digestion of this micro-algae with anaerobic sludge. Please...
03 March 2018 3,668 10 View
Basically, I am looking at design where I have exogenous Sequence A- Intein N and endogenous Sequence B - Intein C - Endogenous sequence C. Would this strategy work, if I had to obtain the...
03 March 2018 6,333 3 View
02 March 2018 6,474 6 View
A hallmark of cancer cells is defects in the apoptotic cell death program. Apoptosis is a highly regulated cell suicide process that functions to remove unwanted cells. One means of inhibiting the...
27 February 2018 3,229 5 View
What are the differences between stokes radius, radius of gyration and hydrodynamic radius ?? and how can they be measured (size exclusion chromatography, DLS, SAXS) ??
20 February 2018 3,653 5 View
02 February 2018 8,467 6 View
02 February 2018 1,384 8 View
Hi, I am planning to express a membrane protein in a cell-free system. My ultimate goal is to use the protein to immunize a rabbit and obtain antibodies. If i do it without nanodiscs , the...
01 January 2018 8,380 7 View
free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...
15 December 2017 8,024 13 View
12 December 2017 4,790 15 View
For example, protein-protein/nucleic acid/lipid interaction pattern, signal transduction pathway they may participate, or some other else.
12 December 2017 5,256 2 View
Hey Guys, I am calculationg radius of gyration for 1ubq. My reference file is a pdb file. and I cannot change it. So I have to deal with it!! The problem is that I receive 2 warnings: WARNING:...
12 December 2017 5,300 6 View
Hey Guys, I have a ubiquitin, I am planning to claculate radius of gyration for residue 1 to 24 and 25 to 76. How can I make this? What command shall I use? Thank you. Maryam
12 December 2017 3,502 3 View
I am fabricating alphalactalbumin nanotubes via the self assembly process and using the serine protease enzyme. First, I want to assure if after formation the internal cavity has a hydrophobic...
12 December 2017 9,354 0 View
I am trying to deliver a 20kDa protein to cancer mono-layer cells using L4395 Sigma kit ( cholesterol; L-α-phosphatidylcholine and stearylamine). We just have to dissolve this lipid powder in...
11 November 2017 761 4 View
I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...
11 November 2017 9,039 3 View
Hi, I am looking for insights on the keratin quantitative composition in human hair. I am looking to understand if there is a predominant type. I would like to do some in vitro tests with hair...
11 November 2017 6,398 1 View
I've recently experienced some problems in denaturing SDS gels. The sample was boiled and run on a 3% stacking; 10% resolving gel. I obtained some bands in the gel as normal, but in some wells I...
11 November 2017 341 2 View
06 November 2017 1,357 3 View
Hi There, I need to prepare oligomeric amyloid beta for injection into the rodents and Ive seen HFIP is used as amyloid beta solvent in many papers as well as my current protocol. I was wondering...
24 October 2017 8,258 4 View