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Questions related to Proteins
I'm using Waters Acquity UPLC-Synapt G2 Mass spec for the analysis of the whole metabolite from a group of cell lines. The technician provides '.RAW directory' as raw data and '.cdf' file as the...
15 January 2019 4,669 3 View
I am using the "ImageJ" software in order to quantify the protein bands on the SDS-PAGE, but I'm not sure it's the best software for this purpose. Does anyone have the experience of using other...
11 January 2019 3,098 12 View
I need to find an analytical solution for solving the deflection of a simple beam (of viscoelastic materials) based on the materials viscous/elastic property, and the span length.
06 January 2019 3,626 3 View
A sample contains 26 % fat content but after the protease activity the fat content rise up to 53%. Where does the excessive fat come from? Does Protease converting the proteins into...
01 January 2019 6,681 11 View
I am using the "ImageJ" software in order to quantify the protein bands in the SDS-PAGE. but I'm not sure it's the best software for this purpose. Does anyone have the experience of using other...
01 January 2019 7,864 12 View
I am trying to detect PIkBalpha on my blot. The Primary and secondary antibody dilutions prepared were absolutely fresh. O/N incubation with primary antibody after Ihr RT blocking and 1 hr...
01 January 2019 5,047 10 View
Hi to all polymer physicists, is it possible, that E modulus of Jello (or it's German version "Göttersspeisse" - the protein content may differ) is, according to the concentrations of the Jello...
01 January 2019 3,280 4 View
I am wondering if there are any web-based programs, like ExPASy that can "predict" a protein's sequence based on structural input. For example, ExPASy SWISS-MODEL can predict protein structure...
01 January 2019 4,541 13 View
I am trying to recover transgenic fluorescing expressing neurons from adult mouse brain. Papain dissociation system by Worthing was used in first few trials but yield no transgenic fluorescing...
18 December 2018 143 3 View
I've recently run into serious problems with my Western blot protein transfers to NC membrane. When I do the Ponceau stain, I get something as shown in the picture below - white blotches covering...
16 December 2018 2,563 6 View
12 December 2018 2,256 6 View
I have quantified a total protein in a sample and then resolved those proteins on the SDS PAGE. I have got those specific proteins at their respective sites but I want to calculate the percentage...
11 November 2018 4,288 0 View
My sample would be around 100-500ul and I wanna purify His tagged proteins from it.
11 November 2018 763 4 View
I am planning to express a recombinant protein in Ecoli and want it secreted extracellular (into the media). I have been reading that Gaussia's sec signal would work in prokaryotes as well. I...
11 November 2018 2,697 4 View
For my current project I do a lot of research about aaRS but didn't find answers to these questions yet. I am happy about any information about the lifecycle of these enzymes.
11 November 2018 5,193 2 View
06 November 2018 2,945 2 View
Hello, I will like to know what is the best database to look for all the post-translational modifications of my protein of interest. I have found Phosphosite Plus...
24 October 2018 6,206 4 View
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23 October 2018 3,481 3 View
I was trying to do membrane leakage assay for one toxic protein. in order to do that I am using E. coli lipid extracts to create liposomes. I am using TbCl3 solution to incorporate the Tb3+ ions...
10 October 2018 7,355 4 View
Demographers estimate that by 2050, the number of people on Earth will reach 10 billion. With such a number of people, the agricultural economy, logistics of food supplies and people's eating...
10 October 2018 3,624 4 View
Hi all, I am having difficulty in identifying post-translational modification in the protein I am working with. So any suggestions will be highly appreciated. I am working with an enzyme that...
25 September 2018 5,822 4 View
i am interested in examining 5-HT1A receptor upregulation (or down-regulation). I have previously looked at NMDA and AMPA receptors and their phosphorylation state but i am not sure what...
11 September 2018 7,770 0 View
Hi everyone, I am a first year PhD student and I am working on a protein called Thymosin Beta 4 (TB4) and using it exogenously to treat injured cells in the kidney called podocytes. I am mainly...
09 September 2018 3,144 4 View
So I am familiar with CLUSTAL Omega to compare several proteins for conservations with each other. But I need some expertise on how to seclude a bit more special information from comparing several...
08 August 2018 9,306 1 View