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Questions related to Proteins
Hi everyone, I've been decellularizing 2D cell cultures for immunos and LC-MS/MS using 20 mM NH4OH. Basically, I incubate my cells with this solution for 5 min and keep the ECM that is attached...
31 July 2020 3,321 1 View
Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.
30 July 2020 9,457 9 View
Anyone knows what may have gone wrong with those western blots? I initially thought it might be due to air bubbles, but I'm not sure. Someone can help me? Thanks in advance
28 July 2020 4,152 13 View
I have seeded keratinocytes in 6 well plate. 120 ul of RIPA buffer(with Pro. K) was used to break cells on ice. However, Bradford reagent (Biorad, diluted with water , 1:4) did not develop any...
18 July 2020 9,282 1 View
I'm doing a Bradfod assay but currently I'm still confused as to what blank i should use for my sample, which is the supernatant of PAO1 culture grown in AB minimal medium. For the standard, i use...
11 July 2020 2,955 9 View
I am interested in potentially using commercially available customized HPC molecular dynamics simulations. However, I cannot find pricing information on the websites of the vendors. Could someone...
08 June 2020 9,247 3 View
It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...
06 June 2020 6,773 1 View
comparing between two protein with the same molecular mass and different shape .
23 March 2020 9,143 4 View
What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...
10 March 2020 8,227 8 View
Currently I am trying to find out which proteins apart from chlorophyll are broken down or relocated during leaf senescence to explain seasonal changes in carbon, nitrogen, oxygen and hydrogen in...
06 March 2020 2,157 3 View
I want to extract membrane+cytosolic proteins for western blots. I'm working with hardy bacteria, so I have to bead beat for full lysis. During bead beating, the manufacturer protocol recommends...
04 March 2020 4,829 20 View
I want to measure different ions (i.g. Ca2+, Mg2+, Zn2+) in a complex proteins mixture with the MS. However, in the protein mixture some ions will most likely be bound to proteins. I want to free...
28 February 2020 3,430 3 View
I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...
25 February 2020 7,540 4 View
I need to see that how a missense mutation effect the protein structure or function of wild type protein? Thanks.
09 February 2020 4,480 5 View
I’m trying to find a method to embed a transmembrane protein into a SLP but I’m not sure where to start. I’ve thought about conjugating lipid around proteins and having them form a particle but it...
26 January 2020 4,135 2 View
I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...
07 January 2020 3,580 14 View
I want to know why colorless area was observed in the blue plaques?
20 December 2019 7,837 1 View
Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...
15 December 2019 6,816 3 View
Hello, I would like to find whether two proteins have a common interaction path in the graph using the STRING database. There is software that is doing it?
02 December 2019 266 3 View
I have always used other reagents now I bought this one but I am not sure if there is an alternative protocol and I cannot find a lot of information online. Many thanks
27 November 2019 6,616 1 View
i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria?...
17 November 2019 5,045 2 View
Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....
11 November 2019 2,586 5 View
Some proteins change their conformation based on protonation state of particular residues like aspartic acid. Some kinases are examples for this. For example, in case of Abl kinase, protonated...
11 November 2019 5,419 5 View
An uncharged protein (P) has an Asp in position 23 with a molecular weight of 6501 Da, as determined by mass spectrometry. The uncharged mutant of this protein (P') contains a single amino acid...
07 November 2019 10,124 3 View