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Questions related to Proteins
I am working with ARPE19 cells and have been conducting mito stress tests using seahorse technology to determine the optimum H2O2 concentration for inducing cellular damage, however, my basal...
21 January 2024 3,944 1 View
cotton seed oil
23 October 2023 863 0 View
Hello everyone, I'm a PhD student in Ecology and at the moment I'm working on the characterization of cohort of G. insensibilis. Part of this activity involves an NMR for a metabolomic analysis...
25 July 2023 6,402 4 View
Many methods call for reconstituting experimental samples in methanol/ water, but diluting the standards with some sort of plasma (typically blank bovine plasma). Why not dilute the standards in...
29 June 2023 2,432 4 View
I have 1 mg of avidin and 100 mg of biotin. How do I prepare the stock solution? I want to study the interaction of these two using electrochemical workstation? Is 1:1 ratio followed?
19 June 2023 3,379 1 View
Hi everyone, I'm looking for very adherent cell lines for a couple of experiments. There seems to be little information online. In my experience, HEK < Huh7 < Vero With Vero being...
30 May 2023 1,876 5 View
Has anyone encountered this message when starting Xcalibur? Home Page: "The application has failed to create an instance of the compliancedatabase service. It will be shut down to comply with 21...
22 May 2023 4,853 0 View
Conceptualise organizational culture ?
10 May 2023 3,909 7 View
Why the color of p.pastoris medium changed after induction? it becomes a little green, not the yellow as the beginning.
08 May 2023 9,249 1 View
I am trying to perform Label-Free Quantification in Proteome Discoverer 3.0. I am comparing sample files, I set the provided Processing and Consensus Workflows . For Grouping & Quantification I...
13 April 2023 599 1 View
I had run homology modelling on unknown protein structure using I-Tasser and Robetta. Both are consensus that the mutation on the protein is at the loop part of the protein. However, the structure...
09 April 2023 8,409 1 View
For isotopic study of bone sample for diet and trophic level reconstruction, I need to remove inorganic carbon from bone. For the same I need to treat bone samples in acid, (I am using HCl). But...
07 March 2023 4,109 0 View
Hello, I am looking for a docking server that can handle metalloproteins with and without a post-translational modification. I know HADDOCK can handle this but I don't know if any others exist...
01 March 2023 3,878 0 View
One of my publications appeared online first with an error in one of the authors name. The journal made the correction, but the RG website did not. How can we fix it?
12 February 2023 7,032 2 View
is it possible to calculate photoelectron Circular dichroism (PECD) using quantum chemistry?
10 January 2023 1,652 4 View
I am working on cell-cell communication in GBM cancer, and I would like to study the gradient concentration of some factors, specifically I want to detect that gradient formation between cells....
30 November 2022 9,218 0 View
Particularly for human brain tissue
29 November 2022 7,961 0 View
I want to do amino acid analysis in the seed meal of cotton. Can anybody suggest me the best method?
29 November 2022 4,585 0 View
growth kinetics of Actinomycetes?
25 November 2022 9,212 1 View
My protocol requires the establishment of clonal cell lines through the expansion of single cell clones sorted in individual wells by FACS. This is notoriously challenging for fibroblast cell...
24 October 2022 8,491 2 View
Vienna-PTM web server (http://vienna-ptm.univie.ac.at): a toolkit for MD simulations of protein post-translational modifications.
03 October 2022 3,226 0 View
I am doing a study on how a certain exposure may affect (change) biomarker profiles in human serum, i.e. at baseline compared to 1 hour after the exposure. In order to detect any change, I assume...
27 September 2022 6,491 4 View
Outcome depends on the type of food it takes right ? So if a cow consumes Genetically modified grass rather than normal will the quality of milk increase? And can we observe any changes in the...
28 August 2022 3,703 3 View
Recovery analysis is done for the evaluation of sensors as a ratio of estimated concentration and spiked concentration, expresses in percentage. I have came across many articles mentioning...
23 June 2022 4,847 0 View