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Questions related to Proteins
i am a post graduate student who i am writing on project topic prevalent and predictors of postpartum depression i need good and recent publications and books for how do i access it?
25 March 2024 2,957 1 View
I'd appreciate any recommendations for a small nonspecific DNA binding protein to use as a tag. We wish to use DNA as a binding scaffold to assemble a protein polymer. Hopefully something with a...
18 March 2024 5,861 2 View
Hi, I have a peculiar problem which persists, so my strains BL21DE3 and Rosetta both have my GOI cloned in pET vectors with Ampicillin and Kanamycin resistance. I have grown them in shakeflasks...
15 March 2024 2,978 6 View
I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of 60%water/30%methanol/10% acetic acid, the chemistry reverses and I lose the...
14 March 2024 9,460 3 View
my protein His-Sumo-insert (Theoretical pI: 5.84), is a plant protein, and it's getting expressed in ecoli, but everything was going into pellet fraction. i have tried changing cells (BL21,...
14 March 2024 2,210 3 View
Hello everyone, I am having trouble with my cell culture. I have used the same cells in the passt, but now every time I thaw a batch (no matter if my own stocks or the original stocks of the lab)...
13 March 2024 6,867 1 View
I have some trouble while using the Emulsiflex B15. Since last week, if i turn the air control knob to fill the sample inside the homogenizer, the sample does not enter well. And even if it...
05 March 2024 7,681 1 View
Hey all, I performed Nuclear protein extract isolation (as well as chromatin bound protein) and diluted it too much. Is there anyway I can concentrate my nuclear protein extract again?
04 March 2024 6,590 3 View
Munsell Color System #@
27 February 2024 2,334 2 View
Alkali Blue 6B and Acid Blue 119 are the same compound in the measurement of Acidity in edible oil and fat? If not, color range by pH for those compounds is different? e.g. pH 9.4 (blue-violet)...
05 February 2024 5,785 0 View
In a Western Blot (WB) experiment, does the concentration of SDS-PAGE gel affect the position of protein bands? In the image, the same protein samples were used for all five lanes. The left side...
30 January 2024 6,660 3 View
I am working with ARPE19 cells and have been conducting mito stress tests using seahorse technology to determine the optimum H2O2 concentration for inducing cellular damage, however, my basal...
21 January 2024 4,041 1 View
cotton seed oil
23 October 2023 935 0 View
Hello everyone, I'm a PhD student in Ecology and at the moment I'm working on the characterization of cohort of G. insensibilis. Part of this activity involves an NMR for a metabolomic analysis...
25 July 2023 6,465 4 View
Many methods call for reconstituting experimental samples in methanol/ water, but diluting the standards with some sort of plasma (typically blank bovine plasma). Why not dilute the standards in...
29 June 2023 2,558 4 View
I have 1 mg of avidin and 100 mg of biotin. How do I prepare the stock solution? I want to study the interaction of these two using electrochemical workstation? Is 1:1 ratio followed?
19 June 2023 3,445 1 View
Hi everyone, I'm looking for very adherent cell lines for a couple of experiments. There seems to be little information online. In my experience, HEK < Huh7 < Vero With Vero being...
30 May 2023 2,020 5 View
Has anyone encountered this message when starting Xcalibur? Home Page: "The application has failed to create an instance of the compliancedatabase service. It will be shut down to comply with 21...
22 May 2023 4,947 0 View
Conceptualise organizational culture ?
10 May 2023 3,994 7 View
Why the color of p.pastoris medium changed after induction? it becomes a little green, not the yellow as the beginning.
08 May 2023 9,307 1 View
I am trying to perform Label-Free Quantification in Proteome Discoverer 3.0. I am comparing sample files, I set the provided Processing and Consensus Workflows . For Grouping & Quantification I...
13 April 2023 674 1 View
I had run homology modelling on unknown protein structure using I-Tasser and Robetta. Both are consensus that the mutation on the protein is at the loop part of the protein. However, the structure...
09 April 2023 8,525 1 View
For isotopic study of bone sample for diet and trophic level reconstruction, I need to remove inorganic carbon from bone. For the same I need to treat bone samples in acid, (I am using HCl). But...
07 March 2023 4,174 0 View
Hello, I am looking for a docking server that can handle metalloproteins with and without a post-translational modification. I know HADDOCK can handle this but I don't know if any others exist...
01 March 2023 3,930 0 View