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Questions related to Proteins
I'm trying to decide which baseline (sigmoidal or linear) would fit better to calculate the melting enthalpy. What could be considerend as a "significant" shift in the baseline (before and after...
02 August 2018 4,497 3 View
I have three relative ratios of potassium (K) to nitrogen (N) = 1 i.e., K= 0.8, K=1.0, K=1.2. Leaf photosynthesis increased substantially by increasing K ratio. While, seed cotton and...
26 July 2018 5,054 6 View
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
24 July 2018 1,516 4 View
I have extracted and purified a few protein from plant leaf. Which is the best and easy method to identify these proteins? I also want to know which gene is responsible for translation of these...
21 July 2018 4,356 7 View
07 July 2018 8,777 3 View
Hello, what is currently the best de novo motif/domain search tool or workflow, if we start from user provided set of protein sequences in FASTA? I have used MEME suite (MEME, DREME, GLAM2) for...
07 July 2018 1,884 5 View
Hi all, I am going to carry out some experiment about protein asorption on my titanium scaffolds. i want to use the SEM XPS in order to detect N picks, whic are most likely associated to...
07 July 2018 4,864 4 View
I am looking for a biomimetic material to simulate the sorbent properties of proteins. It is crucial that the extraction of adsorbed compounds from this material is as easy as possible. Has anyone...
07 July 2018 5,055 7 View
Question: does the Rosetta force field incorporate amino acid post-translational modifications such as hydroxyproline and C-terminal amidation? If yes, where can I see this in the database? If...
19 June 2018 1,803 2 View
Chemical covalent bond investigation between phospholipids and protein??
06 June 2018 1,544 3 View
I would like to understand the reason why proteins show frothing.
06 June 2018 2,880 7 View
Hello, I am wondering if could be possible to align set of protein sequences (for example 100 protein sequences) each to each by any user friendly way. I.e. sequence no. 1 with the sequence no....
05 May 2018 9,090 11 View
Hello all, I'm looking for amino acid, peptide or protein based inducers in gene expression and the repressors they bind to, also is there any database available for this?
05 May 2018 8,964 0 View
Hello, I would like to ask if somebody know user friendly way to blast query sequence (protein or nucleotide) against custom (user-defined) database of sequences. Ideally to work on Windows...
05 May 2018 5,330 4 View
Hello, I am interested in obtaining set of all human proteins with the DNA binding ability in FASTA format in one step. Downloading one by one is very tedious for me :-D Do you know any easy...
05 May 2018 4,638 5 View
I need to isolate the membrane proteins to evaluate the effect of a treatment on this cell fraction.
05 May 2018 4,157 5 View
Hello, Do you have a robust method to get multiple charges for proteins in MALDI-TOF? I would like to improve the precision on the mass for big proteins. I work with a UltraFleXtreme from...
05 May 2018 8,505 2 View
Hello community, Is it possible to store my lysate for protein purification at 4 degrees (or -20 degrees) for a few days, or a week, if the proteins are enzymes which I want to use for further use.
05 May 2018 5,544 0 View
I am researching an O/W emulsion, which is obtained from fractionated black soldier fly larvae. The emulsion is very stable and I know it contains protein, so I hypothesize that the proteins...
19 April 2018 9,965 3 View
04 April 2018 9,826 3 View
I am looking for protocols for extraction of NATIVE unknown proteins from a plant source. The proteins from the specific plant are not reported in literature. At the moment I am using a phosphate...
04 April 2018 6,501 6 View
Hello everyone! I am using Plumed-2.2.2 patched with Gromacs-5.1.2 (GPU version) to run a WT-MetaD for a peptide-bilayer system. The three CVs I use are: (1.) bilayer-peptide distance in...
04 April 2018 9,467 1 View
Hello all. I currently have an issue with N-terminal truncated impurities of desired protein. E. coli has been designed to express the desired protein for analysis and the process has been...
22 March 2018 3,575 4 View
Protein folding/misfolding mechanism has been explored since the 1950s. I would like to know some of the challenges in this domain.
16 March 2018 188 4 View