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Questions related to Proteins
Hello everyone :) I have a question. Does anyone know which is the best antibody to use against dCas9-VP64 fusion protein or just VP64?Thank you a lot in advance :)
07 June 2017 1,749 2 View
I have the list of proteins which are either inhibited or induced by action of certain small molecule. I would like to know the downstream effects of this action so that I can hypothesise a mode...
06 June 2017 2,143 4 View
I lysed the tissue of miceto analyze the soluble and insoluble fraction of total proteins with NP-40 lysis buffer. But my insoluble fractions became gelly after adding sample buffer and I couldn't...
06 June 2017 8,380 4 View
my protein was not expressed on the eukaryotic expression plasmid in vitro？
06 June 2017 8,391 2 View
In an experimental condition (e.g. high concentration of cytotoxic drug) there occurs more than 90% cell death, then is it possible to get equal b-actin (or other loading control protein) as in...
06 June 2017 7,646 0 View
Hi, I'm in bioindustry company. We use Sepharose Q FF for purification of our target protein. But recently, the peak was lower than before and the recovery also decreased. I think all of the...
06 June 2017 5,929 6 View
Hello I am working on a recomnibant protein, after its solublization i refolded the protein by using buffers of different composition and pH. In order to determine which buffer is resulting in the...
06 June 2017 4,303 3 View
[E]: 10 ug/mL Vmax: 0.098 U/mg Protein mw: 128 kDa Reaction volume: 150 uL I'm having trouble getting to S-1 units for Kcat determination. Kcat = Vmax/[E] so I should divide 0.098 U/mg by 10...
06 June 2017 2,476 0 View
tried rosetta but didnt get expression.
06 June 2017 5,455 3 View
I have a Pichia expressed and secreted protein, which i need isolate. Anyone knows how to purify the protein from the media in a large volume setting?
06 June 2017 9,147 2 View
I need bacterial and virus antigen from bacteria and virus infected leaves, respectively. Can i use any protein extraction method for this purpose irrespective of pathogen type? I am confused that...
06 June 2017 9,540 5 View
I am planning to extract total protein from tumor tissue and to coat it as antigen for Indirect ELISA application. Please let me know what buffer I should use to dissolve the protein so that it...
06 June 2017 888 6 View
06 June 2017 1,297 1 View
Currently, we are investigating the effect of protein and fat on sludge dewatering process. We are not sure whether it is possible or not and wonder that is there any expertise who could determine...
06 June 2017 6,917 2 View
For the expression of subtle Nus tag fusion protein I have optimized sonication for 5ml culture, 50 percent amplitude, 1pulse off 1pulse ON for 30 second. With repeated cycles with the gap of...
06 June 2017 9,114 10 View
Greetings I have difficulty understanding the challenges of comparing different membranes during WB analysis. I am looking at the degree of phosphorylation of proteins, but they cannot be stripped...
06 June 2017 9,146 9 View
Hello friends, During proteomic analysis of stem cell secretome, we observed that secretome is significantly enriched for PFAM protein domain (kinesin motor domain). I was also wondering about...
06 June 2017 938 4 View
As part of an assay, I need to re-suspend acetone precipitated protein samples from whole heart tissue in a 1 mL RIPA buffer that has 1% SDS. I'm having great difficulty breaking up the tissue, so...
06 June 2017 5,805 5 View
Basically I have now two proteins that catalyze the same reaction, but with different affinity for their substrate. The first protein has a lower Km since is enough to visualize the reaction...
06 June 2017 4,001 8 View
Quenching studies with phosphoinositides.
06 June 2017 9,253 0 View
Hello, Is it possible to separate a complex fluid in a vesicular part (containing vesicles / exosomes) and a non - vesicular part, containing other proteins? Many thanks!
06 June 2017 2,882 4 View
After DSF guided refolding of protein, results for denatured and refolded samples are coming out like this. is it ok if the denatured sample give peak and Tm like this? as i have used mild...
06 June 2017 8,720 4 View
I am obtaining low yield when purifying IgG from patients with lupus. Any troubleshoot advise?
06 June 2017 4,077 2 View
Any research paper on DSF guided refolding of novel protein ? other then this one https://www.nature.com/articles/srep18906.pdf Thanks Regards
06 June 2017 9,750 0 View