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Questions related to Proteins
Hi, I am working on a practical involving protein digestion and I am confused as to how there can be a -19 Da difference. I believe it has something to do with the trypsin digestion and its...
15 September 2020 5,268 4 View
I am using cbioportal and I put a query about specific genes in several studies on different cancer types. In some studies there are charts of mRNA expression compared between different genes: how...
20 August 2020 3,421 1 View
I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...
17 August 2020 7,452 8 View
Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...
07 August 2020 6,521 5 View
Hi, can anyone guide me with this query of; In order to obtain apo-form of α-LA, is by adding 3-5 mM EGTA to the solution of holo-α-LA (Ca2+bound). This solution was dialysed against the several...
04 August 2020 4,696 3 View
Hi everyone, I've been decellularizing 2D cell cultures for immunos and LC-MS/MS using 20 mM NH4OH. Basically, I incubate my cells with this solution for 5 min and keep the ECM that is attached...
31 July 2020 3,611 1 View
Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.
30 July 2020 9,708 9 View
Anyone knows what may have gone wrong with those western blots? I initially thought it might be due to air bubbles, but I'm not sure. Someone can help me? Thanks in advance
28 July 2020 4,405 13 View
I have seeded keratinocytes in 6 well plate. 120 ul of RIPA buffer(with Pro. K) was used to break cells on ice. However, Bradford reagent (Biorad, diluted with water , 1:4) did not develop any...
18 July 2020 9,479 1 View
I'm doing a Bradfod assay but currently I'm still confused as to what blank i should use for my sample, which is the supernatant of PAO1 culture grown in AB minimal medium. For the standard, i use...
11 July 2020 3,144 9 View
i have an Autolab PGSTAT302N potentiostat
05 July 2020 6,198 3 View
When using UPLC-Q-TOF-MS, the mass spectra of some standards has a ion of [2M+85], I want to know what is the structure the [85] represents? I attach the mass spectrum of Diisopropyl phthalate...
27 June 2020 9,148 0 View
usually inducers of tau protein aggregation cause aggregation gradually within few days , is there any exceptions for this ?
12 June 2020 7,984 0 View
I am interested in potentially using commercially available customized HPC molecular dynamics simulations. However, I cannot find pricing information on the websites of the vendors. Could someone...
08 June 2020 9,528 3 View
Could you please inform me how much patchdocking is reliable in terms of docking of protein – protein interaction before and after mutation with some deleterious amino acid substitutions. In...
08 June 2020 5,272 1 View
It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...
06 June 2020 7,012 1 View
I am working on investigating the secondary structure of protein aggregates using CD. However, my sample is gel-like or non-transparent samples. I was thinking of dilution the sample (H2O)...
19 May 2020 1,798 0 View
I would like to determine if my protein of interest has the post-translational modification of O-link-glycosylation at a specific site. Can I use native mass spec to determine that or should I use...
11 May 2020 5,604 0 View
dear colleague, thanks for your marvellous paper :Sports Hernias: Experience in a Sports Medicine Center. I currently study sex ratio in pubalgia. could you give precisions about sex ratio in...
17 April 2020 1,794 0 View
comparing between two protein with the same molecular mass and different shape .
23 March 2020 9,324 4 View
What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...
10 March 2020 8,612 8 View
Currently I am trying to find out which proteins apart from chlorophyll are broken down or relocated during leaf senescence to explain seasonal changes in carbon, nitrogen, oxygen and hydrogen in...
06 March 2020 2,320 3 View
I want to extract membrane+cytosolic proteins for western blots. I'm working with hardy bacteria, so I have to bead beat for full lysis. During bead beating, the manufacturer protocol recommends...
04 March 2020 4,998 20 View
I want to measure different ions (i.g. Ca2+, Mg2+, Zn2+) in a complex proteins mixture with the MS. However, in the protein mixture some ions will most likely be bound to proteins. I want to free...
28 February 2020 3,585 3 View