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Questions related to Proteins
dear colleague, thanks for your marvellous paper :Sports Hernias: Experience in a Sports Medicine Center. I currently study sex ratio in pubalgia. could you give precisions about sex ratio in...
17 April 2020 1,683 0 View
comparing between two protein with the same molecular mass and different shape .
23 March 2020 9,203 4 View
What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...
10 March 2020 8,408 8 View
Currently I am trying to find out which proteins apart from chlorophyll are broken down or relocated during leaf senescence to explain seasonal changes in carbon, nitrogen, oxygen and hydrogen in...
06 March 2020 2,209 3 View
I want to extract membrane+cytosolic proteins for western blots. I'm working with hardy bacteria, so I have to bead beat for full lysis. During bead beating, the manufacturer protocol recommends...
04 March 2020 4,896 20 View
I want to measure different ions (i.g. Ca2+, Mg2+, Zn2+) in a complex proteins mixture with the MS. However, in the protein mixture some ions will most likely be bound to proteins. I want to free...
28 February 2020 3,482 3 View
I am currently working on a project where I need to recover and purify a number of His-tagged proteins for multiple 30 L fermentation runs. At smaller scales (around 1 L ferms) I have used a high...
26 February 2020 4,290 3 View
I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...
25 February 2020 7,654 4 View
It is well known that acid and alkali treatment helps in deproteinizing the animal bones and fish scale by removing the collagen and other organic matters. But what exact process take place during...
22 February 2020 437 0 View
I need to see that how a missense mutation effect the protein structure or function of wild type protein? Thanks.
09 February 2020 4,505 5 View
I need help in knowing what atomic bonds/stretching/bending each peak occurred and how can it relate to the chemical structure of BSA? If you have a picture of the chemical structure of BSA, that...
09 February 2020 9,028 4 View
I’m trying to find a method to embed a transmembrane protein into a SLP but I’m not sure where to start. I’ve thought about conjugating lipid around proteins and having them form a particle but it...
26 January 2020 4,238 2 View
I would like to ask the above question in context of intact protein characterization. Is it possible to detect DTT in positive mode using 0.1% formic acid gradient, and in which conditions?
15 January 2020 1,413 3 View
I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...
07 January 2020 3,660 14 View
I want to know why colorless area was observed in the blue plaques?
20 December 2019 7,873 1 View
Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...
15 December 2019 6,899 3 View
Hello, I would like to find whether two proteins have a common interaction path in the graph using the STRING database. There is software that is doing it?
02 December 2019 315 3 View
I have done an amplitude sweep of cell suspensions ranging from 0.01-100% strain by an Anton par interfacial rheometer. At lower strain, I am getting lower elastic modulus less than 0.001 Pa. at...
28 November 2019 1,811 2 View
I have always used other reagents now I bought this one but I am not sure if there is an alternative protocol and I cannot find a lot of information online. Many thanks
27 November 2019 6,663 1 View
i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria?...
17 November 2019 5,111 2 View
Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....
11 November 2019 2,651 5 View
Some proteins change their conformation based on protonation state of particular residues like aspartic acid. Some kinases are examples for this. For example, in case of Abl kinase, protonated...
11 November 2019 5,500 5 View
An uncharged protein (P) has an Asp in position 23 with a molecular weight of 6501 Da, as determined by mass spectrometry. The uncharged mutant of this protein (P') contains a single amino acid...
07 November 2019 10,222 3 View
01 November 2019 967 4 View