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Questions related to Proteins
Hello, I am interested in obtaining set of all human proteins with the DNA binding ability in FASTA format in one step. Downloading one by one is very tedious for me :-D Do you know any easy...
05 May 2018 4,551 5 View
I need to isolate the membrane proteins to evaluate the effect of a treatment on this cell fraction.
05 May 2018 4,100 5 View
Hello, Do you have a robust method to get multiple charges for proteins in MALDI-TOF? I would like to improve the precision on the mass for big proteins. I work with a UltraFleXtreme from...
05 May 2018 8,436 2 View
Hello community, Is it possible to store my lysate for protein purification at 4 degrees (or -20 degrees) for a few days, or a week, if the proteins are enzymes which I want to use for further use.
05 May 2018 5,496 0 View
I am researching an O/W emulsion, which is obtained from fractionated black soldier fly larvae. The emulsion is very stable and I know it contains protein, so I hypothesize that the proteins...
19 April 2018 9,911 3 View
04 April 2018 9,789 3 View
I am looking for protocols for extraction of NATIVE unknown proteins from a plant source. The proteins from the specific plant are not reported in literature. At the moment I am using a phosphate...
04 April 2018 6,465 6 View
Hello everyone! I am using Plumed-2.2.2 patched with Gromacs-5.1.2 (GPU version) to run a WT-MetaD for a peptide-bilayer system. The three CVs I use are: (1.) bilayer-peptide distance in...
04 April 2018 9,392 1 View
Hi there I have been doing microplate bradford assays on protein samples (they are relatively crude). I have used BSA as a std and each time the standard curve is consistent in slope and...
03 March 2018 4,051 5 View
I have a 1mg liofilized stock of a membrane protein prepared in PBS, but for my next experiments i'll need the protein to be prepared in other buffer. How could I change the buffer without loosing...
03 March 2018 6,325 4 View
Why do protocols use TFA to stop the guanidination reaction?
03 March 2018 9,716 7 View
In all literature I read the cyanylating agent 1-cyano-4-dimethylaminopryidinium tetrafluoroborate (CDAP) is used for cross-linking to sugars, in these we obviously have our hydroxyl oxygen...
03 March 2018 717 2 View
I have green micro-algae (Enteromorpha) powder and want to measure the carbohydrates and proteins before and after the anaerobic digestion of this micro-algae with anaerobic sludge. Please...
03 March 2018 3,613 10 View
Basically, I am looking at design where I have exogenous Sequence A- Intein N and endogenous Sequence B - Intein C - Endogenous sequence C. Would this strategy work, if I had to obtain the...
03 March 2018 6,304 3 View
02 March 2018 6,398 6 View
A hallmark of cancer cells is defects in the apoptotic cell death program. Apoptosis is a highly regulated cell suicide process that functions to remove unwanted cells. One means of inhibiting the...
27 February 2018 3,167 5 View
What are the differences between stokes radius, radius of gyration and hydrodynamic radius ?? and how can they be measured (size exclusion chromatography, DLS, SAXS) ??
20 February 2018 3,585 5 View
02 February 2018 8,402 6 View
02 February 2018 1,294 8 View
Hi, I am planning to express a membrane protein in a cell-free system. My ultimate goal is to use the protein to immunize a rabbit and obtain antibodies. If i do it without nanodiscs , the...
01 January 2018 8,305 7 View
free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...
15 December 2017 7,895 13 View
12 December 2017 4,721 15 View
For example, protein-protein/nucleic acid/lipid interaction pattern, signal transduction pathway they may participate, or some other else.
12 December 2017 5,201 2 View
Hey Guys, I am calculationg radius of gyration for 1ubq. My reference file is a pdb file. and I cannot change it. So I have to deal with it!! The problem is that I receive 2 warnings: WARNING:...
12 December 2017 5,228 6 View