If opposite charges attract each other, then why do neutrons and protons bind with each other, whereas electrons surround them in an atom?

According to science, like charges repel each other and opposite charges attract each other. But if you observe the structure of an atom, neutrons(neutral), and protons(positive) bind tightly with...

23 November 2020 8,881 7 View

What are the effects of chemical fertilizers on human health ?

Can any fertilizer causes cancer ?

06 September 2020 8,217 11 View

How can one determine the lifetime of a compound if quantum yield is known?

I determined the quantum yield of my compound from fluorescence instrument. So can i determine the total lifetime of my compound from this data?

19 August 2020 521 1 View

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04 July 2020 9,111 0 View

How can i ensure the complete oxidation of graphite before quenching the oxidation process by H2O2?

I am following KMnO4 -H2SO4 reaction media for the synthesis of graphene oxide(GO) by an improved process.

04 June 2020 508 3 View

Can anyone help me to know whether band gap of a semiconductor can be changed by loading dye sensitizer or not?

1- n-type semiconductor has an absorption spectra 2-Dye has an absorption spectra 3- Semiconductor+ Dye has an absorption spectra 4- Can I calculate the band gap of of SC, Dye and SC+Dye using...

27 March 2020 4,222 4 View

What is a multi-label classification and how to use it on classifying text documents?

I want to know how multi-label classification work in detail. At the same time, how does the confusion matrix for multi-label classification is constructed? How does the performance measures such...

28 December 2019 8,065 3 View

What are the Model fit parameters for multigroup analysis in AMOS?

I have used SEM with AMOS for my data for hypothesis testing and the model parameters came out good. I wanted to conduct a multigroup analysis with the same sample by categorizing them into two...

16 December 2019 7,453 3 View

Can I use XBridge C18 ( 3x150mm, 3.5 um) column in a Aquity UPLC system for method development?

I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.

15 December 2019 8,433 12 View

How many different origin of replication (ori) sequences can I have in a plasmid?

Multiple ori sequences pertaining to different hosts are usually present in a plasmid. But how many different ones can I have, without causing problems? If problems do get created, what kind of...

25 November 2019 400 2 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How can I analyze the morphology of microglia (ramification, braches, soma, etc.) via Fiji/ImageJ?

Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...

31 January 2021 3,855 3 View

How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

How to make MRI image segmentation and create binary mask with imagej?

I have a number of MRI Dicom images. I need to create a binary mask with ROI for feature extraction. How I can do this by imagej?

25 December 2020 8,396 2 View