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Questions related to Proteins
We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...
31 July 2024 2,967 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
30 July 2024 9,277 2 View
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25 July 2024 3,103 3 View
I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...
16 July 2024 5,171 2 View
Greetings! I would like to visit the United States, New England area, around December - January. Please let me know with regard to any research conference related to Digital governance,...
11 July 2024 1,817 0 View
I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...
05 July 2024 9,636 0 View
Detail procedure of primer designing?
01 July 2024 2,062 3 View
Hi everyone, I ran a Generalised Linear Mixed Model to see if an intervention condition (video 1, video 2, control) had any impact on an outcome measure across time (baseline, immediate...
15 June 2024 2,159 4 View
What are the specific techniques used by these two countries, i need to do a comparative analysis of these two countries
31 May 2024 7,415 0 View
Pour l'Afrique centrale d’où je suis les populations restent en marge des activités économiques formelles qui impulserait le développement du fait du manque de culture financière. Un marché...
30 May 2024 5,170 0 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
30 May 2024 4,667 0 View
Hi, there are 2 bands in my protein's MW. What could be the reason for shifting the band from the original band? My protein's molecular weight is 131 KDa and it is bound with a FAD cofactor. I...
29 May 2024 4,308 1 View
Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted...
30 April 2024 3,577 4 View
Hello! I am working on investigating the effect of freeze and thaw on the peptides stability. The peptide is relatively short less than 50 AA so my question is would this have an impact at all? Is...
08 April 2024 7,538 4 View
We used the wrong protocol and used all the plate wash and dye release reagents, but still have more of everything else. I wanted to see what to use so the rest of the kit doesn't go to waste.
03 April 2024 6,753 1 View
i am a post graduate student who i am writing on project topic prevalent and predictors of postpartum depression i need good and recent publications and books for how do i access it?
25 March 2024 2,898 1 View
I'd appreciate any recommendations for a small nonspecific DNA binding protein to use as a tag. We wish to use DNA as a binding scaffold to assemble a protein polymer. Hopefully something with a...
18 March 2024 5,780 2 View
Hi, I have a peculiar problem which persists, so my strains BL21DE3 and Rosetta both have my GOI cloned in pET vectors with Ampicillin and Kanamycin resistance. I have grown them in shakeflasks...
15 March 2024 2,876 6 View
I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of 60%water/30%methanol/10% acetic acid, the chemistry reverses and I lose the...
14 March 2024 9,375 3 View
my protein His-Sumo-insert (Theoretical pI: 5.84), is a plant protein, and it's getting expressed in ecoli, but everything was going into pellet fraction. i have tried changing cells (BL21,...
14 March 2024 2,134 3 View
Hello everyone, I am having trouble with my cell culture. I have used the same cells in the passt, but now every time I thaw a batch (no matter if my own stocks or the original stocks of the lab)...
13 March 2024 6,794 1 View
I have some trouble while using the Emulsiflex B15. Since last week, if i turn the air control knob to fill the sample inside the homogenizer, the sample does not enter well. And even if it...
05 March 2024 7,581 1 View
Hey all, I performed Nuclear protein extract isolation (as well as chromatin bound protein) and diluted it too much. Is there anyway I can concentrate my nuclear protein extract again?
04 March 2024 6,489 3 View
Alkali Blue 6B and Acid Blue 119 are the same compound in the measurement of Acidity in edible oil and fat? If not, color range by pH for those compounds is different? e.g. pH 9.4 (blue-violet)...
05 February 2024 5,707 0 View