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Questions related to Proteins
Understanding the susceptibility of small ruminants to artificial infection will provide insights into improved methods for the 'revival' and 'amplification' of frozen stabilates where mice might...
25 September 2025 8,122 0 View
I am purifying a small heat shock protein that is highly prone to aggregation. To prevent aggregation during purification, I plan to include 200 mM arginine in my buffer. However, for the final...
24 September 2025 7,414 1 View
In osteoarthritis, the M1/M2 balance in the macrophage population is correlated with disease severity. Reprogramming macrophages from M1 to M2 by inducing efferocyctosis is being considered as a...
06 August 2025 1,475 0 View
I if there might be any opportunities to collaborate or assist in any ongoing research work. I’m eager to contribute, learn, and support wherever possible.
05 August 2025 6,495 7 View
Hi everyone, I want to use an oily adjutant for my vaccine formulation, but to confirm its sterility, I need to perform an indirect sterility test using the filtration method. In fact, my oily...
30 July 2025 3,407 1 View
monomers were both of hydrophobic and hydrophilic nature. emulsion was stabilized using emulsifiers anionic and non-ionic
22 April 2025 7,471 2 View
I am studying the relationship between Adversity Quotient and Emotional Quotient among Adolescents. I am trying to look for Adversity Response Profile specific for young people ages 12-16 y.o.
14 April 2025 6,232 2 View
In agriculture, AI technologies are increasingly used for tasks like crop monitoring and yield prediction. However, inconsistent data quality can introduce biases, affecting decision-making. It's...
27 September 2024 8,245 3 View
We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...
31 July 2024 3,079 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
30 July 2024 9,448 2 View
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25 July 2024 3,475 3 View
I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...
16 July 2024 5,403 2 View
Greetings! I would like to visit the United States, New England area, around December - January. Please let me know with regard to any research conference related to Digital governance,...
11 July 2024 1,929 0 View
I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...
05 July 2024 9,787 0 View
Detail procedure of primer designing?
01 July 2024 2,105 3 View
Hi everyone, I ran a Generalised Linear Mixed Model to see if an intervention condition (video 1, video 2, control) had any impact on an outcome measure across time (baseline, immediate...
15 June 2024 2,298 4 View
What are the specific techniques used by these two countries, i need to do a comparative analysis of these two countries
31 May 2024 7,542 0 View
Pour l'Afrique centrale d’où je suis les populations restent en marge des activités économiques formelles qui impulserait le développement du fait du manque de culture financière. Un marché...
30 May 2024 5,345 0 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
30 May 2024 4,857 0 View
Hi, there are 2 bands in my protein's MW. What could be the reason for shifting the band from the original band? My protein's molecular weight is 131 KDa and it is bound with a FAD cofactor. I...
29 May 2024 4,413 1 View
Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted...
30 April 2024 3,784 4 View
I'm looking for free or subscription apps to make my statistical analysis. Even online apps would be useful. I used to have MiniTab on Windows but it isn't compatible with MacOS. Thanks!
27 April 2024 5,725 1 View
Hello! I am working on investigating the effect of freeze and thaw on the peptides stability. The peptide is relatively short less than 50 AA so my question is would this have an impact at all? Is...
08 April 2024 7,642 4 View
We used the wrong protocol and used all the plate wash and dye release reagents, but still have more of everything else. I wanted to see what to use so the rest of the kit doesn't go to waste.
03 April 2024 6,841 1 View