Proteins

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Questions related to Proteins

Can recovery percent value be more than 100?

Recovery analysis is done for the evaluation of sensors as a ratio of estimated concentration and spiked concentration, expresses in percentage. I have came across many articles mentioning...

23 June 2022 4,816 0 View

Is it normal for a compound to possess more than one CCS?

I was evaluating the collision cross section (CCS) values for various compounds using traveling wave ion mobility spectrometry (TWIMS). Quite beyond my expectation, some compounds seem to possess...

31 December 2021 6,598 10 View

Can anyone provide a FID chromatogram or elution order of the following fatty acids?

I'm looking for the elution order of the following fatty acids in C18:1n9t, C18:1n9c, C18:1n7, C24:1, C22:4n6, C22:5n3, C22:5n6, and C22:6n3. I use Supelco SP 2560 Capillary GC Column.

02 December 2021 1,217 3 View

Biomarkers for AD?

I am working on looking for a biomarker for Alzahimer diseases. When I compared the results for treatment cells to basal in AD group and treated cells from healthy group vs basal, I see a...

28 November 2021 4,505 4 View

What are the recommended software for Voronoi diagram analysis in a image ?

I want to study the defectivity of hexagonally packed cylinders. I prefer to use methods like Voronoi diagram analysis or Delaunay triangulation analysis for the defect analysis in the SEM and...

23 November 2021 4,616 1 View

Is it possible to convert mg/L to kg/ha?

I am gathering data from papers and some authors present the availability of nutrients as kg/ha and others as mg/L. Would that be possible to convert mg/L to kg/ha once L is volume and ha is...

27 October 2021 971 14 View

Detection of soluble recombinant protein aggregates by native PAGE?

I have been able to express a soluble 6xHis-tagged protein but purification is extremely inefficient. We are only able to increase our yield by overloading the resin (8-10 ml lysate on 50 ul...

13 September 2021 5,940 3 View

How to remove methionine at the N-terminus of Ovalbumine?

Hello, I ran into a serious problem during my experiments. For some reasons, I need glycine at the N-terminus of my ovalbumin (mammalian cell expression, slightly modified protein, ordered from...

08 September 2021 1,571 3 View

Mcf 7 cells not growing after 48 hours?

After fumigation of co 2 incubator, I thawed the cell line from where I used to thaw before too and after 48 hours media color changed to yellow and turbid. I used rpmi media from another lab and...

23 August 2021 1,019 5 View

Why am I not getting any plaques while doing top agar method?

When I spot the phage lysate on MG1655, I see the clearance. But, to calculate the phage titer I mix the lysate (100ul) with MG1655 as background strain (100ul) in 3ml 0.5% agar. In this case, I...

17 August 2021 7,183 21 View

Why does the band gap of PbO (1.9eV) and SnO (0.7eV) vary so much?

PbO and SnO have the same crystal structure and similar ionicity. Can it be explained by orbital molecular theory?

09 August 2021 438 5 View

When making selection media for a transfection, do I put the antibiotic in media with FBS and PenStrep or fresh media (without any FBS and Penstrep)?

I am working with HepG2 cells and transfecting them with a plasmid that upregulates the GABRA1 gene. I need to put them in selection media before I observe the results. HepG2 cells are resistant...

08 July 2021 2,946 2 View

What is the reason for non-standard DSC baseline shape?

Greetings! We use thermogravimetric analyzer TGA/DSC 3+ (Mettler Toledo) to study clay minerals, soils and other objects. Every single DSC curve we get (including empty alumina crucible, alumina...

05 July 2021 7,440 8 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

05 February 2021 4,670 4 View

Why can I not find all of my residues in model predicted by Swiss-Model?

I am trying to perform homology modeling using the swiss-model but I have found that the swiss-model gives the same number of residues as that of the template. Whereas my sequence has more...

22 January 2021 5,712 3 View

Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

13 January 2021 5,588 2 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

25 November 2020 6,731 7 View

Can I dissolve freeze-dried catalase and lysozyme in de-ionised water or do I need a buffer?

Can I dissolve freeze-dried catalase or lysozyme directly into de-ionised water or do I need to use a buffer (separate solutions, not both proteins in one)? I will not be storing the solutions and...

11 November 2020 3,329 5 View

How are sequence similarity clusters defined in the Protein Data Bank?

Hi, I am new to the field of structural biology and am trying to understand how sequence similarity clusters are defined in the Protein Data Bank. As a non-scientist, I would be grateful for a...

25 October 2020 5,504 3 View

Can anyone explain the difference between recombinant proteins vs recombinant chimeric proteins?

Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests

29 September 2020 5,433 3 View

What causes this -19 Da change?

Hi, I am working on a practical involving protein digestion and I am confused as to how there can be a -19 Da difference. I believe it has something to do with the trypsin digestion and its...

15 September 2020 5,099 4 View

How can I break biotin-streptavidin bond and separate them?

I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...

17 August 2020 7,120 8 View

Is someone used a Single or double point method for protein intrinsic viscosity determination?

Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...

07 August 2020 6,315 5 View

How can we check the real content of calcium in the protein samples?

Hi, can anyone guide me with this query of; In order to obtain apo-form of α-LA, is by adding 3-5 mM EGTA to the solution of holo-α-LA (Ca2+bound). This solution was dialysed against the several...

04 August 2020 4,414 3 View

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