Can anyone provide a FID chromatogram or elution order of the following fatty acids?

More Goutam Kumar Kundu's questions See All

Typical concentrations of glucose transporters in mouse brain?

I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...

21 December 2020 4,647 2 View

How Shall I perform Principle Component Analysis in GROMACS?

I have to perform PCA as a part of my results and analysis of protein-ligand complex docking using GROMACS 2018.8. How shall I perform PCA and interpret the results for the same? Any insights...

13 December 2020 3,121 2 View

How shall I prepare a protein PDB file in a particular pH for docking and simulation?

I wanted to prepare a protein at an alkaline pH for molecular docking as that is the pH I want to perform my in vitro assays in? I prepared my protein at the required pH through H++ server;...

13 December 2020 2,272 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

How to identify GC peaks for FAME mix which do not match reference peaks?

Hello, I am analyzing 37 component FAME mix using GC. I have got 37 peaks and 25 of them show expected order and height. However, others are not in the similar order with the reference for same...

22 November 2020 7,033 4 View

What could be the many interpretations of the Bass Model?

The Bass model has drawn a lot of research over the years and subsequent research has interpreted the model in numerous ways like Lekhvall and Kahlani. I would love to know any other ways the...

03 November 2020 6,434 1 View

I am looking for a suggestion, how to model 3D layer soil profile of pile on Abaqus?

I am working on numerical modelling of the pile on layer soil using Abaqus. I want to do a 3D model of the pile, thus I have used C3D8R solid element to model the soil around the pile. My...

13 October 2020 7,296 3 View

What is the best procedure to extract bast fibre from plant bark having higher lignin content?

I have tried to extract fiber from the plant bark having higher lignin content with all traditional fiber extraction methods as well as treated up to 20% NaOH Solution at 100 Degree C. However...

04 October 2020 8,149 1 View

Which of the five PDB files (DRGFIN.PDB, DRGMMX.PDB, DRGNOH.PDB, DRGPOH.PDB & DRGAPH.PDB) as an output from PRODRG Server do I need to use?

I have some protein-ligand complex to simulate on GROMACS version:unchecked. For preparation of my ligands, I submitted the extracted Ligand.pdb file and submitted it in the Prodrg server, Energy...

01 September 2020 9,154 5 View

How to solve characteristic equation of free vibration of beam using "fzero" function in MATLAB ?

Hello every one, I am solving a Euler-Bernoulli Beam equation for a free vibration system. The beam is supported on an elastic soil. To solve the characteristic equation i have use "fzero"...

11 August 2020 8,649 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Dr Jannathulla R.

C14:0 C14:1 C15:0 C16:0 C16:1 C17:0 C17:1 C18:0 C18:1c C18:1t C18:2t C18:2c C20:0 γC18:3 C20:1 αC18:3 C21:0 C20:2 C22:0 C20:3n6 C22:1 C20:4 C23:0 C20:3n3 C22:2 C24:0 C20:5 C24:1 C22:6

Goutam Kumar Kundu

Thank you. but I am particularly looking for C18:1n7, C225n3 and C225n6.

These are not included in 37 compounds FAMEmix standard.