I am working on looking for a biomarker for Alzahimer diseases.

When I compared the results for treatment cells to basal in AD group and treated cells from healthy group vs basal, I see a significant difference. (Basal vs treated in the same group).

But when I compared the treatment cells from AD with treated cells from healthy group, I see no differences between them ( treated cells from AD vs healthy group).

Could I say that my treatment could be a biomarker because the cells from healthy group almost reach the level of diseases patients? also, because there is significant difference when compared to basal in the same group?

Or I cannot say it because it should be a significant difference between healthy and diseases patients in treated cells if I am looking for a biomarker?

If I cannot say my treatment could be a biomarker, what is the possible way to explain my results when these is no differences?

I hope you understand my question.

Thank you so much for your help.

Arwa