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Questions related to Proteins
Hi. I´m trying to immobilize a lipase in a polypropylene support. Please, is there a process to increase the contact between this carrier and the enzyme?, because due the hydrophobicity of the...
10 October 2017 2,840 8 View
06 October 2017 9,751 7 View
Hi RG community, I run LC-MS/MS experiments on a Thermo VelosPro Orbitrap, and I often need to share the raw data (chromatograms and spectra) with colleagues. Sometimes, devil is in the detail, so...
28 September 2017 9,495 5 View
I am running a 10 ns NVT production run MD simulation of a protein in NAMD. Everything looked fine till 9 ns, but during the end of the simulation, while running the dcd in VMD, it seems like the...
10 September 2017 2,241 12 View
We are working on the ATH complex as a anticoagulant material. after production of the complex, we want to remove the unbounded AT and Heparin. for purification of this complex, the Butyle-agarose...
09 September 2017 939 1 View
09 September 2017 3,520 13 View
What are the important proteins we need to do croEM in cell division, chromosome separation or microtubule role in cell division context study to get detailed insight? Please list them.
09 September 2017 5,069 0 View
I am not talking about ion exchange chromatography. I am talking about soluble proteins in solution which have a certain buffer capacity. When you add resin beads which deionize the solution...
09 September 2017 1,238 3 View
I am trying to concentrate a 43kDa soluble protein expressed in mammalian cells. I tried to concentrate it but the sample went through the column very quickly even at centrifugation speed lower...
09 September 2017 8,169 13 View
If I have a protein with many surface exposed cysteines (i.e. where the surphur is available for disulphide interaction), is there a way of ensuring dimerization of a protein through (only) two...
16 August 2017 1,593 1 View
I am working on an experiment to produce bioethanol using seaweed hydrolysate as a substrate. However, there are two sugars present (Rhamnose and Glucose), the yeast strain I have is Kluyveromyces...
14 August 2017 2,246 7 View
We may need to use concentrations of up to 4M NaCl in our experiment. Has anyone done anything similar to this before? Would SnoopCatcher/SnoopTag be effective? Any other protein couples/...
08 August 2017 4,111 0 View
08 August 2017 3,339 1 View
I am currently working on a purification kind of MAb. Its PI is 6.11. in one step of process I have to decrease its pH near 5.2 because of some considerations. The base of buffer is tris-HCL. data...
08 August 2017 2,193 1 View
Is there a way to calculate the backbone RMSD for two different peptides that have the same number of residues but one of the side chains is different? It should be possible because the two...
08 August 2017 5,564 4 View
Dear All, I want to study the role of an amino acid in a protein by mutating it to another amino acid. How will decide or select, the new amino acid? What are the parameters I need to consider?
08 August 2017 3,195 2 View
I want to digest my alpha synulein protein (monomers and fibrils). After disaggregation of the fibrils and drying via speedvac, I tried resolubilizing the fibril sample with 20ul 8M...
08 August 2017 8,276 4 View
I am working with a pET30a+ construct and my protein is C-terminal His-tagged. My protein is forming inclusion bodies and I need to get a protocol for isolation of a pure protein from this. An...
08 August 2017 2,109 4 View
Hi All, I have been doing a wet transfer for my 10 % gel in a NC membrane for a protein at 42kDa at 100V for 1h with a freshly prepared transfer buffer substituted with 20% methanol. After my...
08 August 2017 5,917 2 View
06 August 2017 1,332 3 View
01 August 2017 5,102 3 View
Why do we use Saccharomyces cerevisiae for insulin production as opposed to Picha pastoris?Surely the hyperglycosylation of S. cervisae would trigger an immunogenic response in humans, P. pastoris...
24 July 2017 7,972 2 View
Read 3 answers by scientists to the question asked by Juan Sempere-Valverde on Jul 18, 2017
18 July 2017 1,486 3 View
Hello, I'm trying to purify a 47 kD fusion protein by loading the supernatant directly on the column but I'm having issues with multiple species in my elutions. The lanes are: ladder,...
14 July 2017 5,391 6 View