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Questions related to Proteins
I am wondering if there are any web-based programs, like ExPASy that can "predict" a protein's sequence based on structural input. For example, ExPASy SWISS-MODEL can predict protein structure...
01 January 2019 4,473 13 View
I've recently run into serious problems with my Western blot protein transfers to NC membrane. When I do the Ponceau stain, I get something as shown in the picture below - white blotches covering...
16 December 2018 2,531 6 View
12 December 2018 2,215 6 View
I have quantified a total protein in a sample and then resolved those proteins on the SDS PAGE. I have got those specific proteins at their respective sites but I want to calculate the percentage...
11 November 2018 4,249 0 View
My sample would be around 100-500ul and I wanna purify His tagged proteins from it.
11 November 2018 707 4 View
I am planning to express a recombinant protein in Ecoli and want it secreted extracellular (into the media). I have been reading that Gaussia's sec signal would work in prokaryotes as well. I...
11 November 2018 2,636 4 View
For my current project I do a lot of research about aaRS but didn't find answers to these questions yet. I am happy about any information about the lifecycle of these enzymes.
11 November 2018 5,132 2 View
06 November 2018 2,889 2 View
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23 October 2018 3,384 3 View
I was trying to do membrane leakage assay for one toxic protein. in order to do that I am using E. coli lipid extracts to create liposomes. I am using TbCl3 solution to incorporate the Tb3+ ions...
10 October 2018 7,286 4 View
Demographers estimate that by 2050, the number of people on Earth will reach 10 billion. With such a number of people, the agricultural economy, logistics of food supplies and people's eating...
10 October 2018 3,544 4 View
Hi everyone, I am a first year PhD student and I am working on a protein called Thymosin Beta 4 (TB4) and using it exogenously to treat injured cells in the kidney called podocytes. I am mainly...
09 September 2018 3,065 4 View
So I am familiar with CLUSTAL Omega to compare several proteins for conservations with each other. But I need some expertise on how to seclude a bit more special information from comparing several...
08 August 2018 9,275 1 View
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
24 July 2018 1,425 4 View
I have extracted and purified a few protein from plant leaf. Which is the best and easy method to identify these proteins? I also want to know which gene is responsible for translation of these...
21 July 2018 4,290 7 View
07 July 2018 8,672 3 View
Hello, what is currently the best de novo motif/domain search tool or workflow, if we start from user provided set of protein sequences in FASTA? I have used MEME suite (MEME, DREME, GLAM2) for...
07 July 2018 1,817 5 View
Hi all, I am going to carry out some experiment about protein asorption on my titanium scaffolds. i want to use the SEM XPS in order to detect N picks, whic are most likely associated to...
07 July 2018 4,819 4 View
I am looking for a biomimetic material to simulate the sorbent properties of proteins. It is crucial that the extraction of adsorbed compounds from this material is as easy as possible. Has anyone...
07 July 2018 5,017 7 View
Chemical covalent bond investigation between phospholipids and protein??
06 June 2018 1,458 3 View
I would like to understand the reason why proteins show frothing.
06 June 2018 2,825 7 View
Hello, I am wondering if could be possible to align set of protein sequences (for example 100 protein sequences) each to each by any user friendly way. I.e. sequence no. 1 with the sequence no....
05 May 2018 8,975 11 View
Hello all, I'm looking for amino acid, peptide or protein based inducers in gene expression and the repressors they bind to, also is there any database available for this?
05 May 2018 8,933 0 View
Hello, I would like to ask if somebody know user friendly way to blast query sequence (protein or nucleotide) against custom (user-defined) database of sequences. Ideally to work on Windows...
05 May 2018 5,262 4 View