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Questions related to Proteins
Hello, I ran into a serious problem during my experiments. For some reasons, I need glycine at the N-terminus of my ovalbumin (mammalian cell expression, slightly modified protein, ordered from...
08 September 2021 1,669 3 View
After fumigation of co 2 incubator, I thawed the cell line from where I used to thaw before too and after 48 hours media color changed to yellow and turbid. I used rpmi media from another lab and...
23 August 2021 1,091 5 View
When I spot the phage lysate on MG1655, I see the clearance. But, to calculate the phage titer I mix the lysate (100ul) with MG1655 as background strain (100ul) in 3ml 0.5% agar. In this case, I...
17 August 2021 7,243 21 View
PbO and SnO have the same crystal structure and similar ionicity. Can it be explained by orbital molecular theory?
09 August 2021 518 5 View
I am working with HepG2 cells and transfecting them with a plasmid that upregulates the GABRA1 gene. I need to put them in selection media before I observe the results. HepG2 cells are resistant...
08 July 2021 3,014 2 View
Greetings! We use thermogravimetric analyzer TGA/DSC 3+ (Mettler Toledo) to study clay minerals, soils and other objects. Every single DSC curve we get (including empty alumina crucible, alumina...
05 July 2021 7,492 8 View
I am wondering how you would know if you have cell debris or protein aggregation in a harvest media sample? The harvest was filtered twice, and went through UFDF and then depth filtration. But the...
01 July 2021 9,223 0 View
I ordered acrylonitrile butadiene (33/67) copolymer emulsion so I can do coagulation and dipping and form a nitrile product on a mould. I was expecting a creamy emulsion to arrive; however, I...
10 June 2021 8,035 2 View
After searching among literature for months, I am not still able to choose a microalga strain for my PhD project. The idea is to extract as many as possible valuable compounds (such as pigments,...
06 May 2021 8,277 4 View
Ystrom found that, at six months postpartum, both partially breastfeeding and exclusively bottle-feeding were significantly related to higher levels of depressive symptoms in postpartum women...
21 April 2021 4,687 3 View
I've been working on microalgae for some years and still I've not found documents about full scale, operating works for the production of biofuels from microalgae.
18 March 2021 5,144 3 View
Demography basically, has to do with the human population in terms of sizes, density, changes which occurs overtime and so on. Demography can have both positive and adverse effects on the...
09 March 2021 2,544 4 View
In our WT CHO cell cultures we often observe large multinuclear features looking like syncytium of some 10-20 cells fused together. What could it be? Would subcloning help to get rid of those...
21 February 2021 196 4 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
05 February 2021 4,913 4 View
In my current research I've done the following: A GEE analysis was run to explore significant predictors associated with severe postpartum depression symptoms across the 6-month postpartum...
25 January 2021 3,555 2 View
I am interested in using the Postpartum Partner Support Scale by Dr Cindy Lee Dennis in my final academic research project. If anyone can please guide me or help me about whether we require...
23 January 2021 4,964 0 View
I am trying to perform homology modeling using the swiss-model but I have found that the swiss-model gives the same number of residues as that of the template. Whereas my sequence has more...
22 January 2021 5,846 3 View
Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...
13 January 2021 5,724 2 View
I heard about the beneficial using of bone broth that is rich in collagen for healing leaky tight junction in gut; but I did not find scientific evidence about it, may you help me to find...
22 December 2020 2,158 2 View
I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...
25 November 2020 6,865 7 View
Can I dissolve freeze-dried catalase or lysozyme directly into de-ionised water or do I need to use a buffer (separate solutions, not both proteins in one)? I will not be storing the solutions and...
11 November 2020 3,436 5 View
Good day everyone, I am starting cell proliferation assay using alamarBlue and according to the protocol I have, 100% reduced form of alamarBlue is used as positive control. My question is, if I...
27 October 2020 3,316 7 View
Hi, I am new to the field of structural biology and am trying to understand how sequence similarity clusters are defined in the Protein Data Bank. As a non-scientist, I would be grateful for a...
25 October 2020 5,568 3 View
Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests
29 September 2020 5,550 3 View