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Questions related to Proteins
Hey Guys, I have a ubiquitin, I am planning to claculate radius of gyration for residue 1 to 24 and 25 to 76. How can I make this? What command shall I use? Thank you. Maryam
12 December 2017 3,432 3 View
I am fabricating alphalactalbumin nanotubes via the self assembly process and using the serine protease enzyme. First, I want to assure if after formation the internal cavity has a hydrophobic...
12 December 2017 9,305 0 View
I am trying to deliver a 20kDa protein to cancer mono-layer cells using L4395 Sigma kit ( cholesterol; L-α-phosphatidylcholine and stearylamine). We just have to dissolve this lipid powder in...
11 November 2017 705 4 View
I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...
11 November 2017 9,005 3 View
Hi, I am looking for insights on the keratin quantitative composition in human hair. I am looking to understand if there is a predominant type. I would like to do some in vitro tests with hair...
11 November 2017 6,353 1 View
I've recently experienced some problems in denaturing SDS gels. The sample was boiled and run on a 3% stacking; 10% resolving gel. I obtained some bands in the gel as normal, but in some wells I...
11 November 2017 268 2 View
06 November 2017 1,311 3 View
Hi There, I need to prepare oligomeric amyloid beta for injection into the rodents and Ive seen HFIP is used as amyloid beta solvent in many papers as well as my current protocol. I was wondering...
24 October 2017 8,226 4 View
Hi. I´m trying to immobilize a lipase in a polypropylene support. Please, is there a process to increase the contact between this carrier and the enzyme?, because due the hydrophobicity of the...
10 October 2017 2,810 8 View
06 October 2017 9,675 7 View
I am running a 10 ns NVT production run MD simulation of a protein in NAMD. Everything looked fine till 9 ns, but during the end of the simulation, while running the dcd in VMD, it seems like the...
10 September 2017 2,168 12 View
We are working on the ATH complex as a anticoagulant material. after production of the complex, we want to remove the unbounded AT and Heparin. for purification of this complex, the Butyle-agarose...
09 September 2017 908 1 View
09 September 2017 3,419 13 View
What are the important proteins we need to do croEM in cell division, chromosome separation or microtubule role in cell division context study to get detailed insight? Please list them.
09 September 2017 5,018 0 View
I am not talking about ion exchange chromatography. I am talking about soluble proteins in solution which have a certain buffer capacity. When you add resin beads which deionize the solution...
09 September 2017 1,203 3 View
I am trying to concentrate a 43kDa soluble protein expressed in mammalian cells. I tried to concentrate it but the sample went through the column very quickly even at centrifugation speed lower...
09 September 2017 8,101 13 View
If I have a protein with many surface exposed cysteines (i.e. where the surphur is available for disulphide interaction), is there a way of ensuring dimerization of a protein through (only) two...
16 August 2017 1,563 1 View
We may need to use concentrations of up to 4M NaCl in our experiment. Has anyone done anything similar to this before? Would SnoopCatcher/SnoopTag be effective? Any other protein couples/...
08 August 2017 4,056 0 View
08 August 2017 3,308 1 View
I am currently working on a purification kind of MAb. Its PI is 6.11. in one step of process I have to decrease its pH near 5.2 because of some considerations. The base of buffer is tris-HCL. data...
08 August 2017 2,139 1 View
Is there a way to calculate the backbone RMSD for two different peptides that have the same number of residues but one of the side chains is different? It should be possible because the two...
08 August 2017 5,506 4 View
Dear All, I want to study the role of an amino acid in a protein by mutating it to another amino acid. How will decide or select, the new amino acid? What are the parameters I need to consider?
08 August 2017 3,123 2 View
I want to digest my alpha synulein protein (monomers and fibrils). After disaggregation of the fibrils and drying via speedvac, I tried resolubilizing the fibril sample with 20ul 8M...
08 August 2017 8,239 4 View
I am working with a pET30a+ construct and my protein is C-terminal His-tagged. My protein is forming inclusion bodies and I need to get a protocol for isolation of a pure protein from this. An...
08 August 2017 2,043 4 View