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Questions related to Proteins
Dear colleagues, I'm trying to adopt the Agilent 6550 QTOF for the Native MS experiment with the mAbs. The issue is that I don't see any large proteins in the High Mass Mode (up to 10 000 m/z)...
09 September 2019 2,480 3 View
I am using E.coli BL21 Plyss for a recombinant protein production. The cells debris becomes too sticky after lysis. I spin down the pellet at 13000 g (that is the maximum available here). The...
08 August 2019 3,670 7 View
I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...
17 July 2019 2,188 6 View
Which method do you find better to compare between 2 samples? what are the thing I should take into account when using either of them (normalization etc.)?
17 July 2019 3,603 2 View
Greetings, So, I am on the last phase of my project and the person who was supposed to look at my RNA seq data is currently busy thus won’t have time to touch it until next week. Hence, I was...
16 July 2019 7,437 4 View
I purified my protein by nickle column with FPLC. My base purification buffer had Tris 50 mM, NaCl 500 mM, Imidazole 40mM, urea 8M, 2Me 5mM at PH 8. I removed urea by gradient and eluted protein...
10 July 2019 161 8 View
07 July 2019 1,634 6 View
07 July 2019 4,680 2 View
Hello, I am currently working with the self-aggregation of Phe-Phe and Tyr-Phe dipeptides. Phe-Phe can be easily dissolved using HFIP, but Tyr-Phe cannot (even DMSO can't dissolve it easily). Do...
07 July 2019 8,226 5 View
Hello everyone, Currently a student in my first year of PhD, I am reaching out to everyone with experience on pull down assays with m7-GTP Agarose beads and/or mass spectrometry. Right now I am...
16 June 2019 7,830 2 View
Dear researchers, I would like to generate standard curve of proteins with different stokes radius using reverse phase HPLC column. Supposedly I should calibrate by running individual proteins...
13 June 2019 667 19 View
06 June 2019 4,031 2 View
I have solid hen egg White lysozyme. It is given in units, 49700 units/mg. I need to make a 30,000 U/ ul stock and then need to make 2 ml of lysis buffer. The final concentration of this has to be...
17 May 2019 1,502 5 View
Hello, I am working on a biophysics core facility where we have developed a service to check among other things the molecular mass of the protein of interest, the presence of contaminants and...
05 May 2019 5,593 17 View
I was wondering someone might have any idea what could cause the result we observe below. We are studying binding between a protein and small molecule ligand. In order to determine the enthalpy...
05 May 2019 3,198 3 View
There are different ways to produce IgG. One is using intact IgG from blood/plasma donors. The other one is using genetically engineered/recombinant methods which use yeast cell as host. Which one...
16 April 2019 3,448 12 View
Also, what is the minimum protein amount required for PTM analysis through mass spectrometry?
06 April 2019 8,010 3 View
I'm working on magnetic molecular imprinting of proteins with chitosan and sodium alginate. At the first step, I coat the fe3o4 nanoparticles with sodium alginate and then coat the result with...
04 April 2019 3,710 7 View
I am attempting to conjugate the Pluronic F127 polymer with the glycoprotein Transferrin (Tf). I oxidized the Pluronic's terminal -OH groups using Jones oxidation and succinic anhydride,...
03 March 2019 9,093 6 View
I have been analyzing 90-amino-acid fragment of a protein using HSQC and triple-resonance (1H-15N-13C) NMR. I know roughly where arginine, lysine, and tryptophan side-chain peaks lie on the HSQC,...
03 March 2019 5,432 3 View
I have a number or desired DNA fragments. After measuring concentration the 260/280 seems good, ranges between 1.8 to 1.95. However, the 260/230 was very low ranges between 0.03 to 0.09 Does that...
26 February 2019 4,429 4 View
Hello, I have little time in the use of a NETZSCH device. Currently I know that the calibration must be done according to the following parameters: gas flow, type of atmosphere, type of crucible...
15 February 2019 2,026 4 View
I will be looking into the sustainability of an algae value chain using Porphyridium as a feedstock. A biorefinery could produce proteins, sugars en phycoerythrin (PE) from the Porphyridium algae....
02 February 2019 4,001 2 View
Hi to all polymer physicists, is it possible, that E modulus of Jello (or it's German version "Göttersspeisse" - the protein content may differ) is, according to the concentrations of the Jello...
31 January 2019 4,492 3 View