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Questions related to Proteins
I am currently working on a project where I need to recover and purify a number of His-tagged proteins for multiple 30 L fermentation runs. At smaller scales (around 1 L ferms) I have used a high...
26 February 2020 4,413 3 View
I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...
25 February 2020 7,759 4 View
It is well known that acid and alkali treatment helps in deproteinizing the animal bones and fish scale by removing the collagen and other organic matters. But what exact process take place during...
22 February 2020 493 0 View
I need to see that how a missense mutation effect the protein structure or function of wild type protein? Thanks.
09 February 2020 4,557 5 View
I need help in knowing what atomic bonds/stretching/bending each peak occurred and how can it relate to the chemical structure of BSA? If you have a picture of the chemical structure of BSA, that...
09 February 2020 9,085 4 View
Hi everyone. I am interested to see what the damage on smaller animal long bones look like when attempting to extract marrow from long bones using percussive techniques. Most experiments and...
29 January 2020 2,177 5 View
I’m trying to find a method to embed a transmembrane protein into a SLP but I’m not sure where to start. I’ve thought about conjugating lipid around proteins and having them form a particle but it...
26 January 2020 4,323 2 View
I would like to ask the above question in context of intact protein characterization. Is it possible to detect DTT in positive mode using 0.1% formic acid gradient, and in which conditions?
15 January 2020 1,492 3 View
I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...
07 January 2020 3,724 14 View
I want to know why colorless area was observed in the blue plaques?
20 December 2019 7,928 1 View
Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...
15 December 2019 6,964 3 View
Hello, I would like to find whether two proteins have a common interaction path in the graph using the STRING database. There is software that is doing it?
02 December 2019 390 3 View
I have done an amplitude sweep of cell suspensions ranging from 0.01-100% strain by an Anton par interfacial rheometer. At lower strain, I am getting lower elastic modulus less than 0.001 Pa. at...
28 November 2019 1,855 2 View
I have always used other reagents now I bought this one but I am not sure if there is an alternative protocol and I cannot find a lot of information online. Many thanks
27 November 2019 6,734 1 View
i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria?...
17 November 2019 5,183 2 View
Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....
11 November 2019 2,774 5 View
Some proteins change their conformation based on protonation state of particular residues like aspartic acid. Some kinases are examples for this. For example, in case of Abl kinase, protonated...
11 November 2019 5,609 5 View
An uncharged protein (P) has an Asp in position 23 with a molecular weight of 6501 Da, as determined by mass spectrometry. The uncharged mutant of this protein (P') contains a single amino acid...
07 November 2019 10,332 3 View
01 November 2019 1,050 4 View
Dear colleagues, I'm trying to adopt the Agilent 6550 QTOF for the Native MS experiment with the mAbs. The issue is that I don't see any large proteins in the High Mass Mode (up to 10 000 m/z)...
09 September 2019 2,587 3 View
I tried to find the best way to assess ground subsidence due to backfilled open cut mine spoil settlement? Thank you very much for any suggestion.
07 September 2019 2,006 3 View
I am using E.coli BL21 Plyss for a recombinant protein production. The cells debris becomes too sticky after lysis. I spin down the pellet at 13000 g (that is the maximum available here). The...
08 August 2019 3,816 7 View
I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...
17 July 2019 2,473 6 View
Which method do you find better to compare between 2 samples? what are the thing I should take into account when using either of them (normalization etc.)?
17 July 2019 3,677 2 View